Wnt5a通过酪氨酸激酶孤独受体2调控甲状腺乳头状癌细胞的增殖及侵袭  

Wnt5a might regulate the invasion and proliferation of papillary thyroid carcinoma cells through receptor tyrosine kinase-like orphan receptor 2

作　　者：陈亮[1] 石东亮[1] 杨文华[1] 冀宏[2] 张亮[1] Chen Liang;Shi Dongliang;Yang Wenhua;Ji Hong;Zhang Liang(The Second Department of Thyroid and Breast Surgery,the Center Hospital of Cangzhou,Hebei Medical University,Cangzhou 061001,China;Department of General Surgery,the 2nd Hospital of Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北省沧州市中心医院甲状腺乳腺外二科,沧州061001 [2]河北医科大学第二医院普通外科冀宏,石家庄050017

出　　处：《中华实验外科杂志》2018年第11期2051-2054,共4页Chinese Journal of Experimental Surgery

摘　　要：目的检测敲低酪氨酸激酶孤独受体2（ROR2）同时过表达Wnt5a对人甲状腺乳头状癌细胞株K1及BCPAP生物学行为的影响。方法应用噻唑蓝（MTT）实验检测敲低ROR2同时过表达Wnt5a对甲状腺乳头状癌细胞增殖能力的影响。应用平板克隆形成实验检测敲低ROR2，同时过表达Wnt5a对甲状腺乳头状癌细胞克隆形成能力的影响。应用Transwell实验检测敲低ROR2同时过表达Wnt5a对甲状腺乳头状癌细胞侵袭能力的影响。结果MTT实验结果显示，对照组的K1及BCPAP细胞在4d检测到的吸光度（A）值（0.452±0.005、0.432±0.003）分别低于Wnt5a组（0.563±0.004、0.567±0.006），高于si-ROR2组（0.404±0.003、0.389±0.005），差异均有统计学意义（t=-30.026、-34.857、14.258、12.773，P=0.000）。共染的K1细胞及BCPAP细胞在4d检测到的A值（0.501±0.005、0.496±0.003）均低于Wnt5a组，差异有统计学意义（t=-16.771、-19.545，P=0.000）。平板克隆形成实验结果显示，对照组的K1细胞及BCPAP细胞克隆的形成数[（101.00±5.43）、（112.00±4.11）个]低于Wnt5a组[（156.00±4.34）、（168.00±4.55）个]，高于si-ROR2组[（62.00±4.45）、（68.00±3.99）个]，差异均有统计学意义（t=-13.704、-15.819、9.622、13.304，P=0.000）。共染组的K1细胞及BCPAP细胞克隆的形成数[（121.00±4.44）、（128.00±3.89）个]低于Wnt5a组，差异均有统计学意义（t=-9.764、-11.574，P=0.001、0.000）。Transwell侵袭实验结果显示，对照组的K1及BCPAP细胞穿过基底膜细胞数为（52.90±2.36）、（68.40±1.01）个，分别低于Wnt5a组[（164.30±4.35）、（87.70±1.28）个]，高于si-ROR2组[（31.30±2.17）、（39.60±1.34）个]，差异均有统计学意义（t=-38.829、-20.502、11.533、29.728，P=0.000）。共染组的K1及BCPAP细胞穿过基底膜细胞数平均为（83.50±1.42）、（65.90±2.11）个，低于Wnt5a组，差异均有统计学意义（t=-31.986、-15.300，P=0.000Objective To detect the effect of receptor tyrosine kinase-like orphan receptor 2 （ROR2） low-expression combining Wnt5a over-expression on the proliferation and invasion abilities of papillary thyroid carcinoma cells. Methods The proliferation ability of papillary thyroid carcinoma cells with ROR2 low-expressed combining Wnt5a over-expressed was detected by methyl thiazol tetrazolium （MTT）. The clone formation ability of papillary thyroid carcinoma cells with ROR2 low-expressed combining Wnt5a over-expressed was detected by colony formation assay. The invasion ability of papillary thyroid carcinoma cells with ROR2 low-expressed combining Wnt5a over-expressed was detected by Transwell. Results MTT assay results showed that the A values detected on the fourth day of the K1 and BCPAP cells of the control groups （0. 452±0. 005, 0. 432±0. 003 ） were significantly lower than those of the Wnt5a groups （ 0. 563±0. 004, 0. 567±0. 006 ） but higher than those of the Si-ROR2 groups （0.404±0.003, 0.389±0.005） （t=-30.026, -34.857, 14.258, 12.773; P=0.000）. The A values detected on the fourth day of the K1 and BCPAP cells of the costained groups （ 0.501±0. 005, 0. 496±0. 003 ） were distinctively lower than those of the Wnt5a groups （ t=- 16. 771, -19. 545 ; P=0. 000）. The colony formation assay results showed that the numbers of K1 and BCPAP cell clones of the control groups （101.00±5.43, 112. 00±4.11） were distinctively lower than those of the Wnt5a groups （ 156. 00±4. 34, 168.00±4. 55） but higher than those of the si-ROR2 groups （62.00±4.45, 68.00±3.99） （t=-13. 704, -15. 819, 9. 622, 13. 304; P=0. 000）. The clone numbers of K1 and BCPAP ceils of the co - stained groups （ 121.00±4.44, 128.00±3.89 ） were significantly lower than those of the Wnt5a groups （t=-9. 764, -11.574; P=0. 001, 0. 000）. The Transwell assay demonstrated that the numbers of K1 and BCPAP cells passing through the basement membrane of the control groups [（52. 90±2. 36）, （68.40±1.

关 键 词：WNT5A 酪氨酸激酶孤独受体2 甲状腺乳头状癌 细胞增殖 细胞侵袭 

分 类 号：R736.1[医药卫生—肿瘤]