内镜逆行性胰胆管造影术对急性胰腺炎大鼠的作用及其对活性氧／c-Jun氨基末端激酶通路的影响  

Effect of endoscopic retrograde cholangiopancreatography on acute pancreatitis in rats and its effect on reactive oxygen species/c-Jun N-terminal kinase pathway

作　　者：金鑫 游建[1] 肖定 何鑫[1] Jin Xin;You Jian;Xiao Ding;He Xin(Department of General Surgery,Wuhan Forth Hospital,Puai Hospital,Tongji Medical College,Wuhan 430034,China)

机构地区：[1]武汉市第四医院华中科技大学同济医学院附属普爱医院肝胆胰疝外科,430034

出　　处：《中华实验外科杂志》2018年第11期2085-2088,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察内镜逆行性胰胆管造影术对急性胰腺炎大鼠的作用及其对活性氧（ROS）／c-Jun氨基末端激酶（JNK）通路的影响。方法选取60只雄性大鼠，分为空白组（尾静脉注射0.1ml的体积分数0.1％胎牛血清）、模型组（尾静脉注射0.1ml的体积分数0.1％胎牛血清）及手术干预组（内镜逆行性胰胆管造影术）各20只。建模后，处死大鼠后，留取外周血、胰腺组织标本，采用免疫组织化学技术检测3组大鼠核凶子-κB（NF-κB）的核转位，胰腺组织切片采用苏木素-伊红（HE）染色法行病理检查，采用酶联免疫吸附试验（ELISA）测定3组大鼠血清肿瘤坏死因子-α（TNF-d）、白细胞介素（IL）-6、IL-1β水平，采用流式细胞分析检测3组大鼠胰腺组织ROS水平变化，采用Western blot检测3组大鼠胰腺组织磷酸化JNK（p-JNK）、JNK变化。结果与模型组（55.9±2.2、79.6±12.6、322.3±28.5、436.8±32.9）比较，手术干预组的NF-κB、ROS、P-JNK、JNK均明显降低（12.3±1.1、61.2±13.2、2551.4±25.9、289.6±36.5），差异有统计学意义（P=0.011、0.015、0.014、0.009）；与模型组（85.6±2.5、58.6±13.6、82.3±12.5）比较，手术干预组的TNF-α、IL-6、IL-1β水平均明显降低（22.3±1.1、10.3±1.2、11.3±2.3），差异有统计学意义（P=0.018、0.012、0.016）。结论ROS／JNK通路的启动会引发一系列炎性反应加重病情，内镜逆行性胰胆管造影术对急性胰腺炎大鼠的治疗作用是通过抑制ROS／JNK通路发挥作用。Objective To study the effect of ERCP on acute panereatitis in rats and its effect on reactive oxygen species （ROS）/c-Jun N-terminal kinase （JNK） pathway. Methods 60 male rats were divided into blank group （intravenous injection of 0.1 ml volume fraction of 0.1% fetal bovine serum） , model group （intravenous injection of 0.1 ml volume fraction of 0.1% fetal bovine serum） and surgical intervention group （endoscopic retrograde cholangiopancreatography） （n=20）. After the rats were killed, the peripheral blood and pancreatic tissues were collected and the nuclear translocation of nuclear factor-κB （ NF-κB） was detected by immunohistoehemistry. The serum levels of tumor necrosis factor-α （ TNF-α） , interleukin （IL）-6 and IL-1β in rat serum were determined by hematoxylin and eosin （HE） staining and flow cytometry respectively. The changes of p-JNK-JNK in pancreatic tissue were detected by flow cytometric analysis. The changes of p-JNK-JNK were detected by Western blotting method. Results Compared with the model group （35.9±2. 2, 79. 6±12. 6, 322. 3±28.5, 436. 8±32. 9） , the NF-κB, ROS, p-JNK, JNK of the operation intervention group （ 12. 3±1. 1, 61.2±13.2, 2551.4±25.9, 289. 6±36. 5） obviously reduced （ P=0.011, 0. 015, 0. 014, 0. 009 ）. Compared with the model group （85.6±2.5, 58.6±13.6, 82. 3± 12.5） , the level of TNF-α, IL-6 and IL-1β of surgical intervention group （22. 3±1.1, 10. 3±1.2, 11.3±2. 3） obviously reduced （ P=0.018, 0.012, 0.016）. Conclusion The activation of the ROS/JNK pathway may cause a series of inflammatory reactions to aggravate the condition. Endoscopic retrograde cholangiopancreatography plays a role in the treatment of acute pancreatitis rats by inhibiting the ROS/JNK pathway.

关 键 词：急性胰腺炎 内镜逆行性胰胆管造影术 活性氧/c-Jun氨基末端激酶通路 炎性反应 

分 类 号：R576[医药卫生—消化系统]