长链非编码RNA在腹膜纤维化中的表达谱变化  

Study on dynamic expression profile of long non-coding RNA in peritoneal fibrosis

作　　者：王玉浔[1] 姜埃利[1] Wang Yuxun;Jiang Aili(Blood Purification Center,the Second Hospital of Tianjin Medical University,Tianjing 300201,China)

机构地区：[1]天津医科大学第二医院泌尿外科,300201

出　　处：《中华实验外科杂志》2018年第11期2089-2091,共3页Chinese Journal of Experimental Surgery

摘　　要：目的应用高通量长链非编码RNA（1ncRNA）芯片检测小鼠腹膜纤维化组织与正常小鼠腹膜组织中lncRNAs表达的差异，探讨lncRNAs与腹膜纤维化的关系。方法将30只C57BL／6雄性小鼠随机分为正常组、4周模型组、6周模型组；模型组给予腹腔灌注4．5％腹膜透析液（广州百特公司）。分别于实验4周和6周后提取小鼠脏层腹膜组织的RNA，进行差异lncRNAs的筛选，用反转录-聚合酶链反应（RT-PCR）法验证差异表达的lncRNAs。结果芯片结果显示2倍以上变化且差异有统计学意义的lncRNAs共有857条。其中，随着纤维化时间的延长，呈显著上调的有186条；显著下调的有231条（P〈0．05）。结论在小鼠腹膜纤维化组织中lncRNAs的表达谱发生了动态变化，且差异显著，提示lncRNAs可能参与了腹膜纤维化发生发展的基因调控。Objective To detect the dynamic expression of long non-coding RNA （lncRNA） in normal and peritoneal fibrosis tissue using microarray, and to discuss the relationship between lncRNA and peritoneal fibrosis. Methods Thirty male C57BL/6 mice were divided into three groups： normal group, model group for 4 weeks,model group for 6 weeks. Two model groups were injected with 4. 5% peritoneal dialysis fluid （Baxter）. RNA was extracted from mice＇s visceral peritoneal tissue at 4 and 6 weeks after the experiment; Then the lncRNAs expression levels were detected by mieroarray in normal group and model groups separately; Finally the differentially expressed lncRNAs were identified by reverse transcriptase-polymerase chain reaction （RT-PCR）. Results There were 857 lncRNAs differentially expressed more than 2 times in peritoneal fibrosis tissue as compared with nomal peritoneal tissue. Among them, with the prolongation of fibrosis time, 186 lncRNAs were significantly up-regulated, and 231 lneRNAs were significant down-regulated （P〈0.05）. Conclusion The expression profile of lncRNAs in peritoneal fibrosis tissue was changed dynamically, and the difference was significant. Suggesting that lncRNA may play a key role in peritoneal fibrosis development and occurrence.

关 键 词：长链非编码RNA 腹膜纤维化 基因芯片 

分 类 号：R572.2[医药卫生—消化系统]