微小RNA-1243在甲状腺乳头状癌组织的表达及其对人甲状腺癌细胞株TPC-1增殖和迁移的影响  

Expression of microRNA-1243 in papillary thyroid carcinoma and its effect on proliferation and migration of human thyroid carcinoma cell line TPC-1

作　　者：底旺[1] 冀宏[1] 李清怀[1] Di Wang;Ji Hong;Li Qinghuai(Department of Thyroid Surgery,the Second Hospital of Hebei Medical University,Shijiazhuang 050003,China)

机构地区：[1]河北医科大学第二医院甲状腺外科,石家庄050003

出　　处：《中华实验外科杂志》2018年第11期2125-2127,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察微小RNA（miRNA，miR）-1243在甲状腺乳头状癌组织中表达及对人甲状腺癌细胞TPC-1增殖和迁移的影响。方法收取手术治疗的甲状腺乳头状癌患者127例，以同一患者的癌旁组织样本作为对照，利用实时荧光定量聚合酶链式反应（RT-qPCR），检测癌及癌旁组织中miR-1243的表达。体外培养甲状腺乳头状癌TPC-1细胞，利用miRNA抑制物敲低miR-1243后，水溶性甲腊化合物（MTS）法检测细胞的增殖能力及迁移小室（Transwell）检测细胞的迁移能力，结果甲状腺乳头状癌组织中miR-1243的相对表达量为1.27±0.10，显著低于癌旁对照组1.88±0.14，差异有统计学意义（t=15．764，P=0．003）；miR-1243表达量与甲状腺乳头状癌淋巴结转移、TNM分期明显相关（P=0．029、0．035），但与患者的性别、年龄和肿瘤大小无明显相关。空白对照和阴性对照组中miR-1243的表达量分别为1．03±0．09，1．21±0．11，显著高于miR-1243抑制物转染组0．27±0．06，差异有统计学意义（t=16．435，P=0．000）；细胞转染miR-1243的增殖能力5．68±0．07显著高于，空白对照及阴性对照组的4．62±0．17和4．89±0．04，差异有统计学意义（t=2．135，P=0．032）．miR-1243抑制物转染组迁移细胞数（45．64±10．31）均多于空白对照组（23．15±8．21）和阴性对照组（21．734-7．87），差异均有统计学意义（t=1．957，P=0．027）。结论miR．1243在甲状腺乳头状癌组织中表达下调，TPC-1细胞中敲低miR-1243后促进细胞的增殖和迁移能力。Objective To investigate the expression of microRNA （miRNA, miR）-1243 in thyroid papillary carcinoma and its effect on the proliferation and migration of TPC-1 cell line. Methods Total 127 patients with papillary thyroid carcinoma were underwent surgery in Department of Thyroid Surgery the Second Hospital of Hebei Medical University from May 2013 to February 2017 were collected. The expression of miR-1243 in cancer and adjacent tissues was detected by using real-time quantitative polymerase chain reaction （RT- qPCR）, miR-1243 was knocked down in in cultured TPC -1 cells by miRNA inhibitor. The ability of cell＇ s proliferation or migration were tested by water-soluble methylene compound （MTS） or Transwell assay. Results The expression of miR-1243 in papillary thyroid carcinoma were 1.27±0. 10 significantly lower than 1.88±0. 14 in the adjacent control group with the difference being statistically significant （ t=15. 764, P=0. 003 ） ; The expression of miR-1243 was related with lymph node metastasis and TNM staging of papillary thyroid carcinoma （ P=0. 029, 0. 035 ）, but have no related with the gender, age and tumor size of the patients. The expression levels of miR-1243 in blank control and negative control group were 1.03±0. 09, 1.21±0. 11 significantly higher than 0. 27±0.06 in miR-1243 inhibitor transfected group with difference being statistically significant （ t=16.435, P=0.000）. The proliferation ability of miR-1243 cells was significantly higher than that of the control group （4.62±0.17 and 4.89±0.04 ）, and there was significant difference （ t=2. 135, P=0.032 ） in the blank control group and the negative control group. The number of migrating cells （45.64±10.31 ） in miR-1243 inhibitor transfection group was significantly higher than that in blank control group （23.15±8.21 ） and negative control group （21.73±7. 87 ） with difference being statistically significant （t=1.957, P=0. 027）. Conclusion The expression of miR-1243 in thyroid papillary carcin

关 键 词：微小RNA-1243 甲状腺乳头状癌 细胞增殖 细胞迁移 

分 类 号：R736.1[医药卫生—肿瘤]