过表达miR-101对卵巢癌SKOV3细胞增殖和迁移的影响及可能机制  

Effects and possible mechanisms of miR-101overexpression on proliferation and migration of ovarian cancer SKOV3 cells

作　　者：孙连碧 曹东华 任忠名 王立华 SUN Lian-bi;CAO Dong-Hua;Ren Zhong-ming;Wang Li-hua(Department of Health Service,No.65176 Unit,Dalian 116017,China)

机构地区：[1]65176部队卫勤教研室 [2]大连市妇幼保健院遗传教研室

出　　处：《解剖学研究》2018年第5期385-389,共5页Anatomy Research

基　　金：辽宁省博士启动基金(No.20111123)

摘　　要：目的探讨miR-101对卵巢癌SKOV3细胞增殖、迁移的影响及可能机制。方法将卵巢癌SKOV3细胞分为miR-101模拟物组、miR-101阴性对照组及空白对照组,将miR-101模拟物转染至miR-101模拟物组细胞,阴性对照组细胞转染无关序列,空白对照组细胞不作任何处理,采用Real time PCR方法验证其转染效率,分别用MTT方法与Transwell实验检测转染后各组SKOV3细胞的增殖与迁移能力变化。采用双荧光素酶报告基因验证Girdin是否为miR-101的靶基因,Real-time PCR与Western blot实验分别检测各组细胞Girdin mRNA和蛋白表达水平。结果与对照组相比,miR-101模拟物组SKOV3细胞miR-101表达水平显著升高(P<0.01)。与对照组相比,miR-101模拟物组SKOV3细胞增殖、迁移能力显著降低(P<0.01)。双荧光素酶报告基因分析结果显示,Girdin为miR-101的靶基因;过表达miR-101后,Girdin mRNA及蛋白表达水平显著降低(P<0.01)。结论 miR-101可能通过下调Girdin的表达抑制卵巢癌SKOV3细胞的增殖、迁移。Objective To investigate the effect and possible mechanism of miR-101 on proliferation and migration of ovarian cancer SKOV3 cells. Methods The ovarian cancer SKOV3 cells were divided into miR-101 mimics group,miR-101 negative control group and control group. The miR-101 mimics were transfected into the SKOV3 cells of miR-101 mimics group,the negative control group cells was transfected with unrelated sequences,and the control group cells were not treated with any treatment. Real time PCR method was used to verify the transfection efficiency. The proliferation and migration ability of SKOV3 cells in each group after transfection were detected by MTT and Transwell,respectively. Double luciferase reporter gene was used to verify whether Girdin was a target gene for miR-101. The expression level of Girdin mRNA and protein in each group was detected by real-time PCR and Western blot respectively. Results Compared with the control group,the expression level of miR-101 in SKOV3 cells of miR-101 mimics group was increased significantly（P〈0.01）.Compared with the control group,the proliferation and migration ability of SKOV3 cells in miR-101 mimics group was decreased significantly（P〈0.01）. The results of the double luciferase reporter gene analysis showed that Girdin was the target gene of miR-101,and the expression level of Girdin mRNA and protein was decreased significantly after overexpression of miR-101（P〈0.01）. Conclusion MiR-101 could inhibit the proliferation and migration of ovarian cancer SKOV3 cells by down regulating the expression of Girdin.

关 键 词：卵巢癌 miR-101 Girdin SKOV3细胞 增殖 迁移 

分 类 号：R737.31[医药卫生—肿瘤]