Anticataractogenic effect of hesperidin in galactose-induced cataractogenesis in Wistar rats  

作　　者：Ramar Manikandan Munuswamy Arumugam 

机构地区：[1]Departmen of Zoology, University of Madras

出　　处：《World Journal of Ophthalmology》2016年第1期1-9,共9页世界眼科杂志

基　　金：Supported by University of Madras under UGC-UPE-Ⅱand DBT-BUILDER program(BT/Prl2047/INF/22/199/2014);University of Madras for the starter grant under DST-PURSE-Ⅱprogram

摘　　要：AIM: To explore the anticataractogenic potential of hesperidin, a flavanone, in galactose-induced cataractogenesis.METHODS: In this study, cataract was induced by administering galactose enriched food in a set of rats. Effect of different dosages of hesperidin(25, 50 and 75 mg/kg body weight) were administered simultaneously with galactose in prevention of cataract was determined in another set. In both sets of animals, the levels of peroxidation, oxidants(NO and OH), antioxidants(enzymatic: Superoxide dismutase, catalase, glutathione S-transferase, GPx and non-enzymatic: Reduced glutathione, vitamin E), aldose reductase and sorbitol were determined in the eye lens. In addition, glucose and lipid peroxidation levels were also tested in serum. The quantitative changes in lens inducible nitric oxide synthase(iN OS) and its expression were also determined using Western blot and real-time polymerase chain reaction analyses.RESULTS: Galactose enriched food produced cataract in both the eye lens as a sequel to elevated serum glucose. Simultaneous administration of hesperidin not only reduced serum glucose but also prevented cataract development, through reduced levels of reactive oxygen species(NO and OH) and i NOS expression as well as elevated enzymic and non-enzymic antioxidants were observed in the eye lens.CONCLUSION: These results indicate the preventive effect of hesperidin against cataract in hyperglycemic rats.AIM: To explore the anticataractogenic potential of hesperidin, a flavanone, in galactose-induced cataractogenesis.METHODS: In this study, cataract was induced by administering galactose enriched food in a set of rats. Effect of different dosages of hesperidin(25, 50 and 75 mg/kg body weight) were administered simultaneously with galactose in prevention of cataract was determined in another set. In both sets of animals, the levels of peroxidation, oxidants(NO and OH), antioxidants(enzymatic: Superoxide dismutase, catalase, glutathione S-transferase, GPx and non-enzymatic: Reduced glutathione, vitamin E), aldose reductase and sorbitol were determined in the eye lens. In addition, glucose and lipid peroxidation levels were also tested in serum. The quantitative changes in lens inducible nitric oxide synthase(iN OS) and its expression were also determined using Western blot and real-time polymerase chain reaction analyses.RESULTS: Galactose enriched food produced cataract in both the eye lens as a sequel to elevated serum glucose. Simultaneous administration of hesperidin not only reduced serum glucose but also prevented cataract development, through reduced levels of reactive oxygen species(NO and OH) and i NOS expression as well as elevated enzymic and non-enzymic antioxidants were observed in the eye lens.CONCLUSION: These results indicate the preventive effect of hesperidin against cataract in hyperglycemic rats.

关 键 词：ANTIOXIDANTS Oxidative stress Galactoseinduced CATARACT Free RADICALS HESPERIDIN Eye lens 

分 类 号：R77[医药卫生—眼科]