Hdm2剪接体促进结直肠癌细胞增殖的分子机制  

Molecular Mechanism of Hdm2 Splice Promoting Proliferation of Colorectal Cancer Cells

作　　者：周锋[1] 沙德胜 刘红[1] ZHOU Feng;SHA De-sheng;LIU Hong(Department of General Surgery,Rugao People＇s Hospital,Rugao,Jiangsu Province,226500 China)

机构地区：[1]江苏省如皋市人民医院普通外科,江苏如皋226500

出　　处：《中外医疗》2018年第30期1-4,共4页China & Foreign Medical Treatment

基　　金：江苏省南通市市级科技计划(指导性)项目(GJZ16037);江苏省南通市卫计委青年科研基金项目(WQ2016096);江苏省如皋市科技计划项目(2016年度第50号)

摘　　要：目的探讨Hdm2剪接体促进结直肠癌细胞增殖的分子机制。方法该研究内容起止时间:2015年1月—2017年12月。构建Hdm2剪接体的稳转细胞株后进行CCK-8的检测,并利用western-blot相关下游蛋白的检测。结果Hdm2剪接体与对照组相比可以促进结肠癌细胞的增殖(P<0.05),p53和p21的表达明显下降(P<0.05),同时上调pp38和p38的表达(P<0.05)。结论 Hdm2剪切体与结肠癌细胞的增殖相关,其通过下调抑癌基因p53,p21的表达,激活p-p38和p38的表达促进结直肠癌的发生和发展。Objective To investigate the molecular mechanism of Hdm2 splice promoting proliferation of colorectal cancer cells. Methods The start and end time of this study： from January 2015 to December 2017. The stable cell line of Hdm2 splice was constructed and tested for CCK-8, and the detection of downstream proteins related to western-blot was used.Results Hdm2 splices promoted the proliferation of colon cancer cells compared with the control group（P〈0.05）, and the expression of p53 and p21 decreased significantly（P〈0.05）, expression of p-p38 and p38（P〈0.05）. Conclusion Hdm2 splicing is associated with the proliferation of colon cancer cells, which promotes the development and progression of colorectal cancer by down-regulating the expression of p53, p21 and activating p-p38 and p38.

关 键 词：结直肠癌 Hdm2剪切体 MRNA P53 

分 类 号：R735.34[医药卫生—肿瘤]