桑黄素对子宫内膜癌AN3-CA细胞凋亡和侵袭的影响  

Effects of Morin on Apoptosis and Invasion of AN3-CA Cells in Patients with Endometrial Cancer

作　　者：陈良凤[1] 王建 王雪梅[3] CHEN Liang-feng;WANG Jian;WANG Xue-mei(Department of Gynecology and Obstetrics,Ankang Central Hospital,Ankang,Shaanxi 725000,China;Department of Gynecology and Obstetrics,Xijing Hospital of PLA Air Force Military Medical University,Xi＇an 710032,China;Department of Gynecology and Obstetrics,the First Hospital of Yulin,Yulin,Shaanxi 718000,China)

机构地区：[1]安康市中心医院妇产科,陕西安康725000 [2]空军军医大学西京医院妇产科,西安710032 [3]榆林市第一医院妇产科,陕西榆林718000

出　　处：《解放军医药杂志》2018年第11期20-23,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家自然科学基金(81172458)

摘　　要：目的探讨桑黄素对子宫内膜癌AN3-CA细胞凋亡和侵袭的影响。方法 MTT法分析不同浓度(10、20、40μmol/L)和不同作用时间(24、48 h)的桑黄素对AN3-CA细胞活性的影响;流式细胞学术分析不同浓度桑黄素对AN3-CA细胞凋亡的影响; Transwell实验分析不同浓度桑黄素对AN3-CA细胞侵袭的影响; Western blotting分析不同浓度桑黄素对AN3-CA细胞基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)表达的影响;结果不同浓度的桑黄素作用于AN3-C细胞,24 h细胞活性高于同浓度48 h的细胞活性(P <0. 05)。AN3-CA细胞的凋亡率随着桑黄素浓度的升高而增加,侵袭能力随着桑黄素浓度的升高而减低(P <0. 05)。AN3-CA细胞中MMP-2和MMP-9蛋白表达随着桑黄素浓度的升高而下降(P <0. 05)。结论桑黄素可通过抑制MMP-2和MMP-9蛋白的表达影响子宫内膜癌AN3-CA细胞凋亡和侵袭。Objective To investigate effects of Morin on apoptosis and invasion of AN3-CA cells in patients with endometrial cancer. Methods Effects of different concentrations （10, 20 and 40 μmol/L） and different action times （24 h and 48 h） of Morin on activity of AN3-CA cells were analyzed by MTT assay. Flow cytometry was used to analyze effects of different concentrations of Morin on apoptosis of AN3-CA cells. Transwell assay was used to analyze effects of different concentrations of Morin on invasion of AN3-CA cells. Western blotting was used to analyze effects of different concentrations of Morin on expressions of matrix metalloproteinase-2 （MMP-2） and MMP-9 proteins in AN3-CA cells. Results In AN3-C cells affecting by different concentrations of Morin, activity at 24 h action was significantly higher than that at 48 h action at the same concentration （ P 〈0.05）. Apoptosis rates of AN3-CA cells were increased with increasing Morin concentrations, while invasion abilities of AN3-CA cells were decreased with increasing Morin concentrations （ P 〈0.05）. Expressions of MMP-2 and MMP-9 proteins in AN3-CA cells were decreased with increasing Morin concentrations （ P 〈0.05）. Conclusion Morin may affect apoptosis and invasion of AN3-CA cells in patients with endometrial cancer by inhibiting expressions of MMP-2 and MMP-9.

关 键 词：子宫内膜肿瘤 桑黄素 细胞凋亡 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]