河南新乡地区汉族人群27个Y-STR基因座遗传多态性检测  

Genetic polymorphism of 27 Y-STR Loci among Xinxiang Han population in Henan Province

作　　者：孟娟 王晓丹 黄艳梅 焦会永 郭利伟 张倩 李茜 MENG Juan;WANG Xiaodan;HUANG Yanmei;JIAO Huiyong;GUO Liwei;ZHANG Qian;LI Xi(Department of Forensic Genetics,School of Forensic Science,Xinxiang Medical University,Xinxiang,Henan 453003;Institute of Criminal Science and Technology,Xinxiang Municipal Public Security Bureau,Xinxiang,Henan 453003;Xinxiang Key Laboratory of Forensic Science Evidence,School of Forensic Science,Xinxiang Medical University,Xinxiang,Henan 453003)

机构地区：[1]新乡医学院法医学院法医物证学教研室,河南新乡453003 [2]新乡市公安局刑事科学技术研究所,河南新乡453003 [3]新乡医学院法医学院新乡市法庭科学重点实验室,河南新乡453003

出　　处：《郑州大学学报（医学版）》2018年第4期452-457,共6页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省教育厅自然科学基金资助项目(13A310849);新乡医学院培育基金重点项目(2013ZDll4)

摘　　要：目的:调查河南新乡地区汉族人群27个Y-STR基因座(包含3个双拷贝基因座)的遗传多态性,为法医学个体识别及法医DNA数据库的建立提供数据。方法:采集263名河南新乡地区汉族无关男性个体血样本,提取基因组DNA,采用AGCU Data Y30六色荧光试剂盒复合扩增,GeneMapper~ID X软件分析结果并分型。结果:DYS392、DYS389Ⅱ、DYS447、DYS522、DYS19、DYS448、DYS481、DYS460、DYS458、DYS635、DYS444、DYS643、DYS557等13个单拷贝Y-STR基因座的基因多样性(GD)大于0.7,DYS527 a/b和DYS385 a/b两个双拷贝Y-STR基因座的GD值大于0.8。在263名无关男性样本中,共检测到261种单体型,其中259种单体型仅出现1次,2种出现2次。27个Y-STR基因座的个体识别力和单体型多样性分别为0.996 1和0.999 9,单体型辨别能力为0.992 4。结论:27个Y-STR基因座组成的单体型在河南汉族群体中具有较高的遗传多态性。Aim： To investigate genetic polymorphism of 27 Y-STR loci（ including three double-copy loci） among Han population in Xinxiang area,Henan province so as to provide basic data for personal identification,paternity identification and forensic DNA database. Methods： The blood samples of 263 unrelated males were collected for extracting genomic DNA. Amplification of 27 Y-STR loci was performed with AGCU Data Y30 6-dye kit,and Gene MapperID X Software was used for genotyping. Results： The gene diversity of 13 Y-STR loci （ DYS392,DYS389 Ⅱ,DYS447, DYS522,DYS19, DYS448, DYS481, DYS460, DYS458, DYS635, DYS444, DYS643, DYS557） was higher than 0. 7,and that of two double-copy Y-STR loci,DYS527 a/b and DYS385 a/b was above 0. 8. A total of 261 haplotypes were found,among which,259 haplotypes were observed only once,2 were observed twice,and the discrimination power,the haplotype diversity and the discrimination capacity were 0. 996 1,0. 999 9 and 0. 992 4,respectively. Conclusion： The haplotypes of 27 YSTR of Han population in Henan have abundant genetic polymorphism.

关 键 词：Y-STR基因座 单体型 遗传多态性 河南汉族 

分 类 号：Q943[生物学—植物学]