高压氧预处理对脑缺血再灌注模型大鼠脑组织HO-1和ALK表达的影响  

Effect of hyperbaric oxygen preconditioning on the expression of HO-1 and ALK in cerebral ischemiareperfusion rats

作　　者：杨春辉[1] 尹雪 邵贵强 吴春荣[1] Yang Chunhui;Yin Xue;Shao Guiqiang;Wu Chunrong(Shanghai Fifth People＇s Hospital Affiliated to Fudan University,Shanghai 200240,China)

机构地区：[1]复旦大学附属上海市第五人民医院,上海200240 [2]上海市闵行区吴泾医院

出　　处：《中华航海医学与高气压医学杂志》2018年第5期310-314,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：上海市闵行区卫生计生委基金资助项目（2016MW02）

摘　　要：目的探讨高压氧预处理（hyperbaric oxygen preconditioning, HBOP）对大鼠脑缺血再灌注后脑组织中血红素氧合酶-1（heme oxygenase-1，HO-1）和间变性淋巴瘤激酶（anaplastic lymphoma kinase，ALK）表达的影响。方法将SD大鼠按照数字表法随机分成假手术组（n＝7）、缺血再灌注组（n＝8）和HBOP＋缺血再灌注组（n＝8）。将HBOP组大鼠置于0.2 MPa、85%氧浓度环境下1 h/d，连续7 d。采用改良的Longa法构建大脑中动脉栓塞（middle cerebral artery occlusion，MCAO）模型，缺血2 h后再灌注，再灌注结束24 h后取材进行TTC染色及qRT-PCR实验检测HO-1、ALK的表达水平。结果HBOP能够显著地降低脑梗死体积比（P〈0.01）。此外，HO-1、ALK表达水平在缺血再灌注组较假手术组明显提高，而HBOP组HO-1和ALK表达水平明显降低，但较假手术组HO-1表达仍有明显的上升（P〈0.05）。结论HBOP能明显降低缺血再灌注大鼠脑组织中HO-1和ALK的表达水平，对缺血再灌注导致的脑组织损伤起到保护作用。Objective To study the effects of hyperbaric oxygen （HBO） preconditioning on the expression of heme oxygenase-1 （HO-1）and anaplastic lymphoma kinase（ALK） in cerebral ischemia-reperfusion （IR） rats.Methods The Sprague-Dawley male rats were randomly divided into 3 groups： the middle cerebral artery occlusion （MCAO） and reperfusion group （n=8）, the sham surgical group （n=7） and the HBO preconditioning ＋ MCAO and reperfusion group （n=8）. The HBO preconditioning rats were exposed to hyperbaric environment at a pressure of 0.2 MPa with 85% oxygen concentration, 1 hour a day, for a succession of 7 days. Modified Longa method was used to develop middle cerebral artery occlusion （MCAO） model and reperfusion was performed 2 hours after ischemia. Twenty-fours after termination of reperfusion, the samples were collected to perform TTC staining, and RT-PCR was used to detect the expression levels of HO-1 and ALK. Results HBO preconditioning could significantly reduce the scope of cerebral infarction（P〈0.01）. Additionally, the expression levels of HO-1 and ALK in the ischemia and reperfusion group were markedly increased, as compared with those of the sham surgical group, while the expression levels of HO-1 and ALK in the HBO group were considerably decreased, however, the expression level of HO-1 was obviously increased as compared with that of the sham surgical group （P〈0.05）.Conclusion HBO preconditioning could significantly decrease the down-regulation of HO-1 and ALK in the brain tissue of ischemia and reperfusion rats, which indicated that it might have a protective effect on the brain damage induced by ischemia and reperfusion.

关 键 词：高压氧预处理 脑缺血再灌注 血红素氧合酶-1 间变性淋巴瘤激酶 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]