尿激酶型纤溶酶原激活物对食管鳞癌细胞增殖与转移的影响  

Effects of urokinase plasminogen activator on proliferation and metastasis of esophageal cancer cells

作　　者：侯磊[1] 雷敏 孙晶莹[3] 霍雪萍[3] 王海鹏[1] HOU Lei;LEI Min;SUNJingying;HUO Xueping;WANG Haipeng(Department of Medical Oncology,Shaanxi Provincial People＇s Hospital,Xi＇an 710068,China;Department of Nursing,Shaanxi Provincial Rehabilitation;Hospital;Department of Central Laboratory,Shaanxi Provincial People＇s Hospita)

机构地区：[1]陕西省人民医院肿瘤内科,西安710068 [2]陕西省康复医院护理部 [3]陕西省人民医院中心实验室

出　　处：《山西医科大学学报》2018年第11期1300-1305,共6页Journal of Shanxi Medical University

摘　　要：目的观察尿激酶型纤溶酶原激活物(urokinase plasminogen activator,u PA)对食管鳞癌ECA-109细胞增殖与转移的影响。方法 MTT法检测ECA-109的u PA干扰细胞(si-RNA)、阴性对照细胞(si-NC)与空白对照细胞(control) 24 h、48 h、72h时OD值。流式细胞术检测细胞周期,迁移与划痕实验检测细胞迁移能力,侵袭实验检测细胞侵袭能力,Western-blot检测细胞VEGF蛋白表达。结果转染后72 h si-RNA组较si-NC组和control组细胞增殖能力明显降低(P <0. 05)。在转染后48h si-RNA组较si-NC组和control组G2期细胞比例明显下降,由(14. 2±2. 6)%与(18. 6±1. 9)%降至(8. 9±1. 2)%(P <0. 05),转染36 h迁移与侵袭细胞数减少(P <0. 05),转染后48 h划痕修复能力降低(P <0. 05)、VEGF蛋白表达下调(<0. 05)。si-NC组与control组相比,各检测指标之间差异无统计学意义(P> 0. 05)。结论在食管鳞癌ECA-109细胞中,u PA的下调能够降低细胞增殖活性,并且抑制细胞侵袭、迁移能力与VEGF蛋白表达,进而抑制其转移能力。Objective To observe the effect of urokinase plasminogen activator（uPA） on the proliferation and metastasis of esophageal squamous cell carcinoma ECA-109 cells. Methods MTT assay was used to detect the OD value of uPA interfering cells（si-RNA） , negative interfering cells（si-NC） and control cells at 24,48, 72 h. Flow eytometry was used to analyze the cell cycle. Migration and scratch experiments were performed to analyze the cell migration ability. Invasion experiments were used to detect the cell invasive ability. Western-blot was used to detect the expression of VEGF protein. Results The cell proliferation ability was significantly lower in si-RNA group than in si-NC group and control group at 72 h after transfeetion（P 〈 0. 05）. The proportion of cells in G2 phase decreased significantly from （ 14. 2 ±2.6） % in si-NC group and （ 18.6 ± 1.9） % in control group to （8.9 ± 1.2） % in si-RNA group at 48 h after transfeetion（ P 〈 0. 05）. Compared with si-NC group and control group, the number of migration and invasion cells decreased in si-ilNA group at 36 h after transfeetion （P 〈 0. 05 ）, the scratch repair ability decreased （P 〈 0. 05 ） and VEGF protein expression was down-regulated at 48 h after transfeetion（ P 〈0.05 ）. There was no significant difference in all indexes between si-NC group and control group （ P 〉 0. 05 ）. Conclusion The inhibition of uPA can reduce the proliferation activity of esophageal squamous cell carcinoma ECA-109 cells, and reduce invasive ability and VEGF expression, thereby inhibit the metastasis.

关 键 词：食管癌 尿激酶型纤溶酶原激活物 细胞增殖 细胞迁移 

分 类 号：R735.1[医药卫生—肿瘤]