补钙对染铅大鼠体内钙锌铁锰镁铜含量的影响  

EFFECTS OF CALCIUM SUPPLEMENTATION ON THE CONTENTS OF Ca,Zn,Fe,Mn,Mg, Cu IN BLOOD AND ORGANS OF RATS EXPOSED TO LEAD

作　　者：赵成萍[1] 王洋[2] 牛瑞燕 程红艳[4] 冯翠萍[2] ZHAO Cheng-ping;WANG Yang;NIU Rui-yan;CHENG Hong-yan;FENG Cui-ping(College of Life Science,Shanxi Agricultural University,Taigu 08031;College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801;Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine,Shanxi Agricultural University,Taigu 08031;College of Resource and Environment,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学生命科学院,太谷030801 [2]山西农业大学食品科学与工程学院,太谷030801 [3]生态畜牧与环境兽医学,太谷030801 [4]山西农业大学资源环境学院,太谷030801

出　　处：《营养学报》2018年第5期465-469,共5页Acta Nutrimenta Sinica

基　　金：国家自然基金项目(No.31201965);山西省自然科学基金项目(No.2010011043-1)

摘　　要：目的通过观察补充不同剂量钙对染铅大鼠血液和脏器中钙锌铁锰镁铜含量的影响,为进一步探索其作用机制,有效预防铅对人体的损害提供理论依据。方法选用21 d龄清洁级SD雄性大鼠60只(50±5)g,随机分为5组,分别为对照组(基础饲料,去离子水)、染铅组(基础饲料,0.2%醋酸铅水)、补钙Ⅰ组(基础饲料添加0.2%钙,0.2%醋酸铅水)、补钙Ⅱ组(基础饲料添加0.4%碳酸钙,0.2%醋酸铅水)、补钙Ⅲ组(基础饲料添加0.8%碳酸钙,0.2%醋酸铅水)。饲养60d后,采集全血、脾脏、肝脏、肾脏、心脏、肌肉,用ICP测定Pb、Ca、Zn、Fe、Mn、Mg、Cu的含量。结果染铅组大鼠血液和脏器中Pb含量均显著或极显著升高(P<0.05或P<0.01);脾脏中Ca、Fe、Zn、Cu,心脏中Ca、Fe、Zn、Cu、Mg、Mn,肝脏中Ca、Fe、Zn、Cu、Mn,肾脏中Ca、Fe、Zn、Cu、Mg、Mn,肌肉中Ca、Fe、Zn、Mg含量等显著下降(P<0.05或P<0.01)。补钙后,血液和各脏器铅含量均下降,其中补钙II组差异显著(P<0.05);补钙组Ca、Zn、Cu、Mg含量在所实验的脏器中均升高,有的差异显著,而Fe在心脏和肝脏中仍有下降,对锰的影响差异不显著。结论补充一定剂量的钙虽然对铅暴露所致的血液和脏器中Ca、Zn、Cu、Mg等元素的异常改变有一定的干预作用,但并不能完全调节由铅所致的所有矿物元素的失衡。因此,需要根据具体情况进行不同矿物元素的补充,以预防铅暴露引起的蓄积性器官损伤。Objective The effects of different doses of calcium on the contents of Ca, Zn, Fe, .Mn, Mg,Cu in the blood and organs of lead exposure rats were observed, which provides a theoretical basis for further investigation on the mechanisms and effectively preventive measures. Methods 60 healthy （50±5g） male SD rats were randomly divided into five groups, as normal control group （basic feed, deionized water） , Pb model group （basic feed, 0.2% lead acetate） , Ca-Ⅰ group （basic feed plus 0.2% calcium, 0.2% lead acetate）, Ca-Ⅱgroup （basic feed plus 0.4% calcium, 0.2% lead acetate） , Ca-Ⅲ group （basic feed plus 0.8% calcium, 0.2% lead acetate）. After 60 days of administration, blood, spleen, liver, kidney, heart and muscle were collected, and the contents of Pb, Ca, Fe, Zn, Mn, Mg, Cu were determined by ICP. Results The contents of Pb in the blood and organs of the rats in the lead group were significantly increased （P〈0.05 or P〈0.01 ） . The contents of some bivalent mineral elements were decreased significantly （P〈0.05 or P〈0.01 ） , such as Ca, Fe, Zn, Cu in the spleen, Ca, Fe, Zn, Cu, Mg, Mn in the heart, Ca, Fe, Zn, Cu, Mn in the liver, Ca, Fe, Zn, Cu, Mg, Mn in the kidney, Ca, Fe, Zn, Mg in the muscle. After calcium supplementation, the levels of lead in the blood and organs were decreased, and the difference for the Ca-II group was significant （P〈0.05） . The contents of Ca, Zn, Cu and Mg in the Ca supplemented groups were increased, and some differences were significant, while Fe contents were decreased in the heart and liver, and the effect on Mn contents was not significant. Conclusion Calcium at a certain dose can intervene the imbalance of to Ca, Zn,Cu,Mg in the blood and organs induced by lead exposure, but can not completely reverse the imbalance. Therefore, specifed measures need to be developed to prevent cumulative organ damage caused by lead exposure according to the specific circumstances.

关 键 词：钙 铅 二价矿物元素 血液 脏器 

分 类 号：R151[医药卫生—营养与食品卫生学]