紫黑色作物花色苷实验室定量检测方法研究  

Quantitative Determination of Anthocyanins from Purple and Black Crops

作　　者：曹婷[1] 刘艳艳[1] 刘兴华[1] 朱明[1] 周步海 杨华[1] 周帅 顾克余[1] Cao Ting;Liu Yanyan;Liu Xinghua;Zhu Ming;Zhou Buhai;Yang Hua;Zhou Shuai;Gu Keyu(Institute of Agricultural Sciences in Coastal Area of Jiangsu Province,Yancheng Jiangsu 224002;Yancheng Yangxu Modern Agricultural Technology Co.,Lid,Yancheng Jiangsu 224002;Yancheng Shine Muscat Grape Plantation Co.,Lid,Yancheng Jiangsu 224005)

机构地区：[1]江苏沿海地区农业科学研究所,江苏盐城224002 [2]盐城市仰徐现代农业科技有限公司,江苏盐城224002 [3]盐城市阳光玫瑰葡萄种植园有限公司,江苏盐城224005

出　　处：《中国农学通报》2018年第30期129-135,共7页Chinese Agricultural Science Bulletin

基　　金：江苏省农业科技自主创新资金项目"优质安全鲜食葡萄产业链关键技术创新与集成应用"[CX(16)1013];江苏省苏北科技专项(富民强县)项目"葡萄优质高效栽培技术集成与示范"(BN2016148);盐城市农业科技创新专项引导资金项目"盐都经济林果观光农业星创天地建设"(YK2016010);江苏省三新工程项目"江苏现代农业(葡萄)产业技术体系盐城推广示范基地"[SXGC(2017)127];江苏沿海地区农科所科研基金项目"花色苷实验室检测结果质量控制方法研究"(YHS201509)

摘　　要：为寻求快速、经济测定自然界中紫黑色作物总花色苷含量的方法,利用酸性乙醇对黑元麦、茄子、葡萄3种作物进行花色苷提取,并采用pH示差法和差减法对3种样品总花色苷含量进行留样分析再测定。结果表明:黑元麦、葡萄采用pH示差法和差减法的结果精确度和准确性没有显著性差异,且样品进行留样再检测时发现,2种样品测定结果之差的绝对值均小于测定结果质量不确定度,说明黑元麦、葡萄花色苷实验室定量检测pH示差法和差减法均适用。茄子采用p H示差法测定值相对标准偏差为2.20%,精确度明显好于差减法;从质控图看,茄子检出值出现1次连续4个点上升的情况,说明茄子皮利用差减法测定结果准确性差;从留样再检测结果可以看出,利用差减法测定2次结果之差的绝对值为0.023 mg/g,大于测定结果质量不确定度0.011 mg/g,说明茄子花色苷pH示差法检测结果可信赖,差减法检测存在问题。To find a rapid and economic method for the determination of total anthocyanins from purple andblack crops in nature, the anthocyanins were extracted from three different crops of black wheat, eggplant andgrape by using acidic ethanol. The total anthocyanin content from the three samples was determined byretentate analysis with pH differential method and subtraction method. The results showed that there was nosignificant difference in the accuracy of the results between the two methods using for black wheat and grapes.It was found that the absolute value of the difference between the results of the two methods was less than theuncertainty of quality determination when the two kinds of samples were re-tested, indicating that pHdifferential method and subtraction method were both applicable to laboratory quantitative detection ofanthocyanin from black wheat and grapes. The relative standard deviation of eggplant measured by pHdifferential method was 2.20%, the precision was better than that of the subtractive method. There was a caseof 4 consecutive points rise for the eggplant anthocyanins detected value from the quality control chart, showing that the accuracy of eggplant peel anthocyanin test results was poor by using subtraction method. The re-testing showed that the absolute value of the difference between the two results obtained by the subtractionmethod was 0.023 mg/g, which was higher than the uncertainty of the measurement result 0.011 mg/g,indicating that the test results of anthocyanin from eggplant by pH differential method were reliable, but thetest results by subtraction method had some problems.

关 键 词：花色苷 PH示差法 差减法 质量不确定度 

分 类 号：S663.1[农业科学—果树学]