区域性角膜内皮损伤动物模型的建立  

Establishment of animal model of regional corneal endothelial cells damaging

作　　者：牛国桢 高佳妮 颜俊卿 刘春雨 张语珊[1] 毕燕龙[1] Niu Guozhen;Gao Jiani;Yah Juaqing;Liu Chunyu;Zhang Yushan;Bi Yaalong(Department of Ophthalmology,Tongfi Hospital Affiliated to Tongji University,Shanghai 200065,China)

机构地区：[1]同济大学附属同济医院眼科,上海200065

出　　处：《中华眼外伤职业眼病杂志》2018年第10期737-740,共4页Chinese Journal of Ocular Trauma and Occupational Eye Disease

基　　金：上海市“科技创新行动计划”实验动物研究领域项目（16140900900）上海市卫生系统优秀学科带头人培养计划（2017BR060）

摘　　要：目的建立重复性好的区域性角膜内皮细胞（CECs）损伤兔眼动物模型。方法依据电脑绘制的CECs损伤区域模拟图，对应兔眼角膜上皮面标记，于透明角膜缘做穿刺口，导入自制刮除器刮除局部CECs，刮除的同时以含低分子肝素的乳酸林格氏液持续灌注前房，以保持前房稳定、清洗刮除的CECs及避免纤维素性渗出所引发的虹膜粘连。结果共7只兔眼，所设计的十字交叉区域性CECs损伤动物模型都顺利完成制作。从制作透明角膜缘穿刺口开始至制模结束，耗时平均（11．82±1．07）min。台盼蓝染色证实CECs刮除范围与制模前角膜上皮面标记平均吻合指数为（94．71±2．17）％。所有模型眼刮除区域的后弹力层组织均保持完好，无因纤维素性渗出而导致模型制作终止或晶状体虹膜损伤的现象发生。结论本研究提出了一种建立区域性CECs损伤动物模型的有效、可控、便捷的方法，且具有较好的可重复性。Objective To establish reproducible rabbit model of regional corneal endothelial cells （CECs） damaging. Methods The border line on the corneal epithelium was marked according to the simulated image of damaged area made by computer. The incision was made on the corneal limbus of rabbit eye, then a self-made scraper was inserted into the anterior chamber. During scraping, Ringer＇ s solution with low molecular heparin was perfused into the anterior chamber persistently, to ensure the stability of anterior chamber, clean the scraped CECs and avoid iris adhesion caused by the fibrous exudation. Results The designed CECs cross damaging models were completed successfully in all 7 rabbit eyes. From making corneal incision to the model made, the time was （ 11.82 ± 1.07 ） minutes on average. Trypan blue staining results showed that the scraped region of CECs was basically consistent with the preoperative corneal epithelium marker, with a consisitent index of （94.71 ± 2.17 ） % on average. The Descemet＇ s membrane of all eyes was intaet. There was no termination during operation due to fibrous exudation, and no len or iris damage occurred. Conclusion The article proposed an effective, controllable and convenient method for establishing an animal model of regional CECs damage, which has good repeatability.

关 键 词：内皮细胞 角膜 动物模型 兔 后弹力层 角膜 

分 类 号：R77[医药卫生—眼科] R-332[医药卫生—临床医学]