半乳糖凝集素3基因在眼部静脉畸形组织中的检测及临床意义  

Detection of Galectin-3 in ocular venous malformation tissue and its clinical significance

作　　者：乔军波 李金 柴昌 王卫群[3] Qiao Junbo , Li din, Chai Chang, Wang Weiqun.(Department of Hemangioma Surgery, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450003, China)

机构地区：[1]郑州大学第三附属医院(河南省人民医院)血管瘤外科,450003 [2]河南省立眼科医院河南省眼科研究所 [3]郑州大学第一附属医院眼科

出　　处：《中华整形外科杂志》2018年第10期867-871,共5页Chinese Journal of Plastic Surgery

基　　金：河南省与国家卫生与计划生育委员会省部共建医学科技攻关项目（201601015）;河南省科技攻关项目（162102310282）

摘　　要：目的检测半乳糖凝集素3（Galectin-3）基因在眼部静脉畸形组织中的表达，探讨其与眼部静脉畸形发生和进展的关系。方法收集12例新鲜眼部静脉畸形组织标本为静脉畸形组，12例单纯性大隐静脉曲张组织近端正常静脉组织标本为对照组，采用实时荧光定量聚合酶链反应技术（Real-time PCR）和Western Blot检测Galectin-3的表达。结果静脉畸形组和对照组Galectin-3mRNA及蛋白的相对表达量分别为5.26×10－3±8.78×10－4、4.89×10－4±5.37×10－5和0.861＋0.394、0.223＋0.206；眼部静脉畸形组织中Galectin-3 mRNA及蛋白水平上的表达量明显高于对照组（P〈0.05），并随着病变侵及深度及范围的加重有递增的趋势。结论Galectin-3 mRNA及蛋白在眼部静脉畸形组织中呈高表达，可能与静脉畸形局部侵袭发生发展和预后密切相关，可用于判断眼部静脉畸形的局部侵犯程度。眼部静脉畸形的发病机制可能与Galectin-3基因的参与有关，待通过基因芯片等技术进行更深入地研究。Objective To detect the expression level of Galectin-3 in the ocular Venous Malformation （VM） tissue, and explore its associations with the occurrence and development of ocular VM.Methods 12 fresh ocular VM tissue samples and 12 simplex great saphenous vein varices tissue samples were collected. The mRNA and protein expression levels of Galectin-3 gene were detected by RT-PCR and Western Blot.Results The relative mRNA and protein levels of Galectin-3 in VM and control group were 5.26×10-3±8.78×10-4, 4.89×10-4±5.37×10-5 and 0.861±0.394, 0.223±0.206, respectively; Galectin-3 expressions in both mRNA and protein levels in ocular VM tissue were obviously higher than those in control group （P〈0.05）, and an increasing trend was accompanied with aggravating pathological changes in both degree and range of ocular VM.Conclusions Galectin-3 is highly expressed in ocular VM tissue in both mRNA and protein levels, which may be closely related to ocular VM development and prognosis, and can be used to determine the degree of local invasion of ocular VM. The pathogenesis of ocular VM may be related to Galectin-3, which can be further investigated through gene chip techniques.

关 键 词：半乳糖凝集素3 眼 静脉畸形 侵袭 聚合酶链反应技术 

分 类 号：R77[医药卫生—眼科]