柘木多酚和氧化白藜芦醇的制备与其抗氧化活性研究  

Preparation and Antioxidant Activities of Polyphenols and Oxyresveratrol in Stems of Cudrania tricuspidata

作　　者：赵建萍 陈倩[2,3] 曹俊东 唐崇明[2] 李雅丽 刘江云[2] ZHAO Jian-ping;CHEN Qian;CAO Jun-dong;TANG Chong-ming;LI Ya-li;LIU Jiang-yun(Health and Social Care Institute,Shanghai Urban Construction Vocational College,Shanghai 201415,China;College of Pharmaceutical Sciences,SooChow University,Suzhou 215123,China;Changzhou Technician Institute,Changzhou 213000,China;Pharmacy Department,the First Peopled Hospital of Yunnan Province,Kunming 650032,China;VIP Internal Medicine Department,the First Affiliated Hospital of Xinjiang,Medical University,Urumqi 830011,China)

机构地区：[1]上海城建职业学院健康与社会关怀学院,上海201415 [2]苏州大学医学部药学院中药系,江苏苏州215123 [3]常州技师学院,江苏常州213000 [4]云南省第一人民医院药学部,云南昆明650032 [5]新疆医科大学第一附属医院VIP内科,新疆乌鲁木齐830011

出　　处：《中国现代中药》2018年第10期1288-1291,共4页Modern Chinese Medicine

基　　金：国家自然基金项目(81460634);宜兴市科技创新(农业)项目(2017NY05)

摘　　要：目的:对柘木多酚及其中主要成分进行制备和抗氧化活性研究,为其开发利用提供参考。方法:柘木茎的75%乙醇提取物采用AB-8大孔树脂精制,对其中的主成分进行分离鉴定和HPLC含量测定,采用DPPH和ABTS法测试其抗氧化活性。结果:柘木茎经精制获得柘木多酚CTP-1、CTP-2;从CTP-1进一步分离获得化合物1,经鉴定为氧化白藜芦醇。HPLC分析表明,二者中均主要含有氧化白藜芦醇。抗氧化活性测验结果表明,CTP-1、CTP-2及氧化白藜芦醇对DPPH自由基的半数抑制浓度(IC50)值分别为19. 1、25. 3、11. 2μg·m L^(-1),对ABTS自由基的IC50分别为49. 3、60. 1、29. 7μg·m L^(-1)。结论:柘木多酚具有强抗氧化活性,氧化白藜芦醇是其主要活性成分。Objective： Polyphenols and key component of Cudrania tricuspidata and their antioxidant capacities were investigated in this study to provide reference data for their potential applications as antioxidant agent in future. Methods： The stems of C. tricuspidata were extracted with 75% ethanol, followed by AB-8 macroporous resin purification to give two frac- tions, CTP-1 and CTP-2, respectively. Key components in CTPs were separated and determined by HPLC. DPPH and ABTS tests were applied to measure their antioxidant activities. Results： Two fractions were thus obtained, and oxyresveratrol（ 1 ）was separated and identified from CTP-1, which was determined to be major ingredient of both CTPs by HPLC. As for antioxidant assay, half inhibition concentrations（ IC50 ）of CTP-1, CTP-2 and oxyresveratrol against DPPH free radicals were 19. 1, 25.3, and 11.2 μg · mL-1, respectively, their IC50 values against ABTS free radicals were 49.3 , 60. 1 and 29. 7 μ ·mL -1 , re- spectively. Conclusion： CTPs possess strong antioxidant capacities, with oxyresveratrol as the major ingredient within them.

关 键 词：柘木 多酚 氧化白藜芦醇 抗氧化 

分 类 号：R283.6[医药卫生—中药学] R285[医药卫生—中医学]