亲水性钛表面微纳米形貌对人牙周膜成纤维细胞增殖与成骨分化的影响  

Hydrophilicity of titanium with micro/nanotopo-graphical surface promotes proliferation and osteogenic differentiation of human periodontal ligament cells

作　　者：林志立[1] 梁建平[1] 庄秀妹[2] LIN Zhili;LIANG Jianping;ZHUANG Xiumei(Department of Stomatology,Guangzhou First People＇s Hospital,Guangzhou 510180,Guangdong Province,China)

机构地区：[1]广州市第一人民医院口腔科,广东广州510180 [2]中山大学附属孙逸仙纪念医院口腔科,广东广州510180

出　　处：《中国口腔种植学杂志》2018年第3期101-104,共4页Chinese Journal of Oral Implantology

基　　金：国家自然科学基金青年基金项目(81600899)

摘　　要：目的:探讨亲水性钛表面微纳米形貌对人牙周膜成纤维细胞(periodontal ligament cells, PDLCs)增殖与成骨分化的影响。方法:前期已通过阳极氧化法和喷砂碱热法构建相似微纳米形貌,但显示不同亲水性的钛表面,两组钛片表面接种PDLCs,采用CCK8法在培养1、4、7、14天时检测PDLCs细胞活性;第7天和14天时检测总蛋白浓度与碱性磷酸酶(ALP)活性,并在第7天时检测成骨标志物ALP、I型胶原(COL1)与成骨特异性转录因子2 (RUNX2)的mRNA表达水平。采用SPSS13.0软件包对数据进行统计学分析。结果:相比阳极氧化组,亲水性更强的喷砂碱热组PDLCs在接种4、7、14天后细胞活性更高;培养7、14天后,喷砂碱热组PDLCs的总蛋白浓度、ALP活性均高于阳极氧化组;第7天时,喷砂碱热组PDLCs中ALP、COL1与RUNX2的mRNA表达水平均高于阳极氧化组。结论:喷砂碱热组钛表面微纳米形貌较阳极氧化组显著促进PDLCs增殖与成骨分化,该过程可能与其良好的亲水性有关。Objective： To investigate the effect of hydrophilicity of titanium with micro/nanotopographical surface on the cell viability and osteogenic differentiation in human periodontal ligament cells（PDLCs）.Methods： Methods of anodic oxidation and sandblast-alkali heat were utilized to establish scale structures. PDLCs were seeded on these two titanium discs, cell viability was detected at 1, 4, 7 and 14 days by CCK8 assay. Total protein values and alkaline phosphatase（ALP） activity were examined at 7 and 14 days. ALP, collagen-I（COL1） and runt related transcription factor 2（RUNX2） m RNA expression at 7 days were detected by qRT-PCR. The date was statistically analyzed with SPSS13.0 software package.Results： Compared with anodic oxidation surfaces, PDLCs on the sandblast-alkali heat surface discs with well hydrophilicity exhibited enhanced cell viability, increased total protein values, up-regulated ALP activity and overexpression of ALP, COL1 and RUNX2 levels. Conclusion： Micro-nanotopographical surface of titanium implant in sandblast-alkali heat group promotes cell viability and osteogenic differentiation of PDLCs, which may be affected by its hydrophilicity.

关 键 词：人牙周膜成纤维细胞 成骨分化 微纳米形貌 亲水性 

分 类 号：R783.1[医药卫生—口腔医学]