发菜基因组DNA提取方法比较与分子鉴定研究  

Comparison and identification of genomic DNA extraction methods from Nostoc flagelliforme

作　　者：陈雪燕[1] 何兵强 谭晴晴[1] 张全芳[1] 汝医[3] CHEN Xueyan;HE Bingqiang;TAN Qingqing;ZHANG Quanfang;RU Yi(Bio-Tech Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,China;Jining University,Qufu 2 73155,China;Institute of Agro-food Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]山东省农业科学院生物技术研究中心,山东济南250100 [2]济宁学院,山东曲阜273155 [3]山东省农业科学院农产品研究所,山东济南250100

出　　处：《药学研究》2018年第11期634-637,共4页Journal of Pharmaceutical Research

基　　金：山东省重点研发计划(No.2018GSF119002);山东省创新公共服务平台计划(No.2018JGX111);山东省农业科学院农业科技创新工程(No.CXGC2018D03)

摘　　要：目的以发菜为研究对象比较不同的提取DNA方法,筛选出了一种适合快速提取发菜DNA的方法。方法分别利用自主研发的硅珠DNA纯化技术、细菌试DNA提取试剂盒和植物DNA提取试剂盒提取发菜基因组DNA。结果本实验室开发的硅珠DNA提取纯化技术具有快速、无毒以及获得的发菜DNA纯度高等优势。通过聚合酶链式反应(PCR)扩增和测序得到16S rRNA基因,利用Blast软件进行序列比对,与发菜同源性为99%。结论本研究研制的发菜基因组DNA提取方法可用于发菜源性的分子鉴定。Objective To select a method for the rapid extraction of Nostoc flagelliforme DNA by comparing different DNA extraction methods. Methods The self-developed silica bead DNA purification technology,the bacterial DNA extraction kit and the plant DNA extraction kit were used to extract the Nostoc flagelliforme genomic DNA,respectively. Results The technology of extraction and purification of silica bead DNA developed by our laboratory has the advantages of rapid,non-toxic and high purity of DNA obtained from Nostoc flagelliforme .The 16S rRNA gene was obtained by PCR amplification and sequencing,and the sequence alignment was conducted by Blast software,with the homology of 99% of the same as Nostoc flagllifome . Conclusion This method can be used for the molecular identification of Nostoc flagllifome.

关 键 词：发菜 DNA提取 16S RRNA 分子检测 

分 类 号：R915[医药卫生—微生物与生化药学]