miR-4286与AEG-1在非小细胞肺癌组织中的表达及临床意义  

Expression of miR-4286 and AEG-1 in non-small cell lung cancer and their clinical significance

作　　者：赵华[1] 陈莹蓉[2] 宋鹏涛[1] 余才华[3] 吴巍[4] 马志红[2] ZHAO Hua;CHEN Yingrong;SONG Pengtao(Department of Pathology,Huzhou Central Hospital,Huzhou 313000,China)

机构地区：[1]湖州市中心医院病理科,313000 [2]湖州市中心医院分子医学重点实验室,313000 [3]湖州市中心医院心胸外科,313000 [4]湖州市中心医院消化内科,313000

出　　处：《浙江医学》2018年第22期2427-2430,2512,共5页Zhejiang Medical Journal

基　　金：浙江省自然科学基金青年项目(LQ14H160015);湖州市公益性技术应用研究一般项目(2013GY17)

摘　　要：目的探讨miR-4286、星形胶质细胞上调基因-1 (AEG-1)在非小细胞肺癌(NSCLC)组织中的表达及与NSCLC临床病理特征的关系。方法分别采用原位杂交方法和免疫组织化学染色检测组织芯片中140例NSCLC癌组织及癌旁正常组织中miR-4286、AEG-1的表达,并分析两者表达水平与NSCLC临床病理特征的关系。结果 NSCLC癌组织中miR-4286高表达率为62.14%,高于癌旁正常组织的4.29%,差异有统计学意义(P<0.01);NSCLC癌组织中AEG-1高表达率为55.71%,高于癌旁正常组织的7.86%,差异有统计学意义(P<0.01)。miR-4286高表达与患者肿瘤直径大小、肿瘤TNM分期和淋巴结转移均有关(均P<0.05),AEG-1高表达与患者肿瘤TNM分期和淋巴结转移均有关(均P<0.05)。Spearman等级相关分析显示NSCLC癌组织中miR-4286的表达与AEG-1的表达呈正相关(r=0.223,P=0.008)。结论 NSCLC癌组织中miR-4286与AEG-1均高表达,miR-4286与AEG-1表达水平与TMN分期和淋巴结转移均有关,提示miR-4286和AEG-1可能参与NSCLC的发生、发展与转移。Objective To investigate the expression of miR-4286 and astrocyte elevated gene-1（AEG-1） in non-small cell lung cancer （NSCLC） and their relationships to clinicopathological features. Methods In situ hybridization and immunohis- tochemistry for miR-4286 and AEG-1 were performed in 140 samples of NSCLC tumor tissues and adjacent normal lung tissues on tissue microarray sections, respectively. The correlation of miR-4286 and AEG-1 expression with clinicopathologic parameters of NSCLC was analyzed. Results The high expression rate of miR-4286 in tumor tissues was 62.14%, which was higher than that in adjacent normal tissues（4.29%） （P〈0.01）. The high expression rate of AEG-1 in tumor tissues was 55.71%, which was higher than that in adjacent normal tissues （7.86%）（P〈0.01）. The upregulated expression of miR-4286 in NSCLC was correlated with tumor size, lymph node metastasis and TNM stage （all P〈0.05）, while the high expression of AEG-1 in NSCLC was associated with TNM stage and lymph node metastasis （both P〈0.05）. Moreover, Spearman analysis showed a positive correlation between miR-4286 expression and AEG-1 expression in NSCLC （r=0.223, P=0.008）. Conclusion The high expression of miR-4286 and AEG-1 in NSCLC is correlated with TNM stage and lymph node metastasis, suggesting that both miR-4286 and AEG-1 may be involved in the occurrence, development and metastasis of NSCLC.

关 键 词：非小细胞肺癌 miR-4286 原位杂交 免疫组织化学 星形胶质细胞上调基因- 1 

分 类 号：R734.2[医药卫生—肿瘤]