血管内皮生长因子C对胃癌淋巴结转移及微转移的预测价值研究  

Predictive value of vascular endothelial growth factor C in lymph node metastasis and micrometastasis of gastric cancer

作　　者：邓庆炎[1] 陈宏[1] 雒洪志[1] 张倩 Deng Qingyan;Chen Hong;Luo Hongzhi;Zhang Qian(The Third Department of General Surgery,Zhongshan City People＇s Hospital,Zhongshan 528400,China)

机构地区：[1]广东省中山市人民医院普外三科,528400

出　　处：《中华普通外科学文献（电子版）》2018年第6期405-408,共4页Chinese Archives of General Surgery(Electronic Edition)

摘　　要：目的分析胃癌患者血清中血管内皮生长因子C(VEGF-C)的表达情况及其与淋巴结微转移的关系,初步探讨VEGF-C对胃癌患者的临床价值。方法选择2012年1月至2016年1月间中山市人民医院行胃癌根治术的患者113例以及同期的健康成人志愿者30例,采用酶联免疫吸附技术(ELISA)检测胃癌患者及健康人群的血清VEGF-C的蛋白质量浓度;免疫组织化学染色法检测常规苏木精-伊红(HE)染色无淋巴结转移的患者淋巴结微转移情况;分析VEGF-C蛋白在血清的表达与患者临床病理资料以及淋巴结微转移之间的关系。结果 113例胃癌患者的血清VEGF-C蛋白质量浓度为(1 687.06±305.21)ng/L,显著高于健康对照的(729.46±182.31)ng/L,差异有统计学意义(t=8.61,P<0.05)。淋巴结转移组66例患者的血清VEGF-C质量浓度为(2 063.75±313.08)ng/L,显著高于无淋巴结转移组47例的(1 208.39±231.92)ng/L,差异有统计学意义(t=7.23,P<0.05)。淋巴结微转移组15例共41枚的VEGF-C质量浓度为(1 306.01±265.69)ng/L,与无淋巴结微转移组32例(1 183.26±206.54)ng/L比较,差异无统计学意义(t=1.92,P>0.05)。不同TNM临床分期的胃癌患者VEGF-C表达水平差异有统计学意义(F=10.21,P<0.05)。以血清VEGF-C质量浓度1 705.60 ng/L为诊断界值,预测淋巴结转移的敏感度和特异度分别为77.3%和83.0%。结论血清VEGF-C浓度可作为胃癌的辅助诊断指标,检测血清VEGF-C浓度对术前预测胃癌淋巴结转移和提高术前临床分期的准确性有益。血清VEGF-C浓度与胃癌淋巴结微转移的相关性不大。Objective ：To investigate the expression of vascular endothelial growth factor （VEGF）-C in serum of patients with gastric carcinoma, and to analyze its relationship and clinicopathological features to predict the clinical value of lymph node metastasis and micrometastasis. Methods：Between January 2012 and January 2016, a total of one hundred and thirteen patients with gastric carcinoma and 30 healthy controls were studied. Serum VEGF-C levels were assessed by enzymed-linked immunosorbent assay （ELISA）. The negative lymph nodes examined by hematoxylin-eosin （HE） staining were analyzed by immunohistochemical staining （IHC） for micrometastasis. Statistical analysis was done to explore the relationship between the expression of VEGF-C protein in serum and clinicopathological data and lymph node micrometastasis. Results？The serum level of VEGF-C protein in patients with gastric cancer was （1 687.06±305.21） ng/L, which was significantly higher than that in healthy controls （729.46±182.31） ng/L （t=8.61, P〈0.05）. The concentration of VEGF-C in serum of 66 patients with lymph node metastasis was （2 063.75±313.08） ng/L, significantly higher than （1 208.39±231.92） ng/L in 47 patients without lymph node metastasis, the difference was statistically significant （t=7.23, P〈0.05）. There was no significant difference in the concentration of VEGF-C between micrometastasis group and non-micrometastasis group [（1 306.01±265.69） ng/L vs （1 183.26±206.54） ng/L, t=1.92, P〉0.05]. The expression of VEGF-C in gastric cancer patients with different TNM clinical stages was significantly different （F=10.21, P〈0.05）. The sensitivity and specificity of predicting lymph node metastasis were 77.3% and 83.0% respectively with serum VEGF-C concentration of 1 705.60 ng/L as the diagnostic threshold. Conclusions：The serum levels of VEGF-C have potential use as diagnostic measurements in gastric carcinoma. Detection of serum VEGF-C concentration is helpful to predict lymph node

关 键 词：胃肿瘤 血管内皮生长因子C 淋巴转移 微转移 

分 类 号：R735.2[医药卫生—肿瘤]