氯化镉对本生烟草幼苗生长及蛋白质表达水平的影响  

Effects of CdCl_2 Treatment on the Growth and Protein Expression Level of Nicotiana benthamiana Seedlings

作　　者：陆玉建 石东里 吴涛 LU Yu-jian;SHI Dong-li;WU Tao(College of Biological and Environmental Engineering,Binzhou University/Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Deha,Binzhou 256603,Shandong,China)

机构地区：[1]滨州学院生物与环境工程学院/山东省黄河三角洲野生植物资源开发利用工程技术研究中心,山东滨州256603

出　　处：《湖北农业科学》2018年第21期87-90,92,共5页Hubei Agricultural Sciences

基　　金：山东省高等学校科技计划项目(J14LE05);滨州学院科研基金项目(2013ZDL04)

摘　　要：为探究氯化镉(CdCl_2)对本生烟草(Nicotiana benthamiana)幼苗生长及蛋白质表达水平的影响,选用不同浓度的CdCl_2处理本生烟草幼苗,测量其根长和茎长,提取本生烟草幼苗总蛋白质,利用SDSPAGE进行检测。试验结果表明,低浓度CdCl_2对本生烟草幼苗的生长具有促进作用;高浓度CdCl_2则抑制了本生烟草幼苗的生长。当CdCl_2浓度达到0.5 mmol/L时,幼苗的生长开始受到抑制;CdCl_2浓度大于1.5 mmol/L时,幼苗大量变黄枯萎。SDS-PAGE显示,低浓度CdCl_2可提高植物总蛋白质的含量,而随着CdCl_2浓度的提高,本生烟草总蛋白质的含量明显下降。In order to investigate the effects of CdCl2 treatment on the growth and protein expression of Nicotiana benthamiana,the seedlings were treated with CdCl2. The growth status of the seedlings was observed and the length of the root and stem was recorded. The protein of Nicotiana benthamiana were extracted by SDS extraction,and isolated by SDS-PAGE. A large number of experimental data showed that low concentration of CdCl2 could promote the growth of the seedlings,while the high concentration of CdCl2 inhibited the growth of the seedlings. When the concentration of CdCl2 reached 0.5 mmol/L,the growth of seedlings began to be inhibited. If the concentration of CdCl2 was greater than 1.5 mmol/L,the seedlings turned yel- low and withered. SDS-PAGE showed that low concentration of CdCl2 could increase the total protein content of the plant,but with the increase of CdCl2 concentration,the total protein content of Nicotiana benthamiana significantly decreased.

关 键 词：CDCL2 本生烟草(Nicotiana benthamiana) 幼苗 总蛋白质 SDS-PAGE 

分 类 号：S572[农业科学—烟草工业]