长链非编码RNA PGM5-AS1在非小细胞肺癌中表达及分子机制  

Expression and molecular mechanism of lncRNA PGM5-AS1 in non-small cell lung cancer tissues

作　　者：张霖 周昌盛 虞晓明 刘瑜[2] 谢奇朋[3] ZHANG Lin;ZHOU Changsheng;YU Xiaoming(Department of Respiratory and Critical Care Medicine,Cangnan County People＇sHospital,Wenzhou 325800,China)

机构地区：[1]苍南县人民医院呼吸与危重症医学科,浙江苍南325800 [2]温州医科大学附属第一医院胸外科 [3]温州医科大学附属第二医院/育英儿童医院检验科

出　　处：《全科医学临床与教育》2018年第6期613-616,共4页Clinical Education of General Practice

基　　金：温州市科技局项目(2017Y0908)

摘　　要：目的探讨长链非编码RNA(lncRNA)PGM5-AS1在非小细胞肺癌中的表达及其作用机制。方法采用实时定量PCR检测26例肺鳞癌和28例肺腺癌的癌组织与正常组织中PGM5-AS1的表达,并利用软琼脂克隆增殖实验检测过表达PGM5-AS1后肺癌细胞的增殖能力。结果 TCGA数据库中PGM5-AS1在肺鳞癌和肺腺癌中表达均较正常组织下调(t分别=3.43、6.27,P均<0.05)。在本次研究肺癌标本库中,无论肺鳞癌还是肺腺癌的癌组织中的PGM5-AS1表达均较正常组织明显下调(t分别=23.72、27.45,P均<0.05)。过表达PGM5-AS1后,H520(PGM5-AS1)及H2170(PGM5-AS1)较对照细胞的细胞集落数明显减少(t分别=8.22、8.19,P均<0.05)。结论 lncRNA PGM5-AS1在肺癌中表达下降,可能通过抑制细胞增殖来发挥抑癌功能。Objective To explore the expression of lncRNA PGM5-AS1 in lung cancer tissues and its molecular mechanism.Methods The expression levels of lncRNA PGM5-AS1 in lung cancer tissues and adjacent normal tissues were detected by quantitative real-time PCR.The proliferation ability of lung cancer cells was detected by cloning and proliferation of soft agar after overexpression of lncRNA PGM5-AS1 in vitro.Results The expression of PGM5-AS1 in lung squamous cell carcinoma and lung adenocarcinoma was down-regulated in the TCGA database when compared with normal tissues（t=3.43,6.27,P〈0.05）.In this study,the expressions of PGM5-AS1 in tumor tissues were significantly lower than that in normal tissues regardless of lung squamous cell carcinoma or lung adenocarcinoma（t=23.72,27.45,P〈0.05）.Moreover,after overexpression of PGM5-AS1,the cell colony amounts of H520（PGM5-AS1）and H2170（PGM5-AS1）were significantly less than those of the control cells（t=8.22,8.19,P〈0.05）.Conclusion The decreased expression of lncRNA PGM5-AS1 in lung cancer may play a role in suppressing cancer by inhibiting cell proliferation.

关 键 词：非小细胞肺癌 长链非编码RNA PGM5-AS1 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]