影响凝胶迁移率实验因素的教学改进  

Improvement of experimental teaching in the influencing factors of electrophoretic mobility shift assay

作　　者：周超 赵翔宇[1] 高新起[1] 郭兴启[1] 苏英华[1] ZHOU Chao;ZHAO Xiang-yu;GAO Xin-qi;GUO Xing-qi;SU Ying-hua(College of Life Sciences,Shandong Agricultural University,Taian 271018,China)

机构地区：[1]山东农业大学生命科学学院,山东泰安271018

出　　处：《实验室科学》2018年第5期4-7,共4页Laboratory Science

基　　金：山东省高等教育名校建设工程项目(项目编号:鲁教高字[2012]14号)

摘　　要：凝胶迁移实验(EMSA-Electrophoretic Mobility Shift Assay)是一种研究蛋白质与核酸相互作用的常用实验技术。在凝胶迁移率实验的教学工作中,由于受实验材料的影响,尤其是EMSA探针浓度的变化,对实验结果的影响很大。为进一步提高实验教学效果,将探针浓度对实验结果的影响进行了比较。通过设置一系列浓度梯度实验,发现采用浓度为5nmol的探针实验效果最好,改进后的实验效果更加明显。Electrophoretic Mobility Shift Assay （EMSA） is a common technique study of interaction between protein and nucleic acid. In the experiment teaching of EMSA, there is a great influence on the results due to the effect of experiment materials, especially the concentration of EMSA probes. In order to improve the experimental teaching effect, the influences of different concentrations of EMSA probes are compared. We found that the probe concentration of 5nmol is best. The improved experiment phenomenon is more obvious.

关 键 词：凝胶迁移率实验 实验教学 探针浓度 

分 类 号：Q-331[生物学]