基于“因地制宜”理论,探讨高寒地区太阴病动物模型的建立  

The Discussion on the establishment of animal model of Taiyin Disease(Syndrome of Deficiency of Spleen Yang) in Alpine Region Based on the Theory of “Local Conditions”

作　　者：李杰[1] 郑靖[1] 陈湘宏[1] 刘占厚[1] 吴萍[1] 张广梅[1] 赵协慧[1] 张发斌[1] 王仁嫒[1] 杨艳[1] 高威 Li Jie;Zhengjing;Chen Xianghong;Liu Zhanhou;Wu Ping;Wang Renai;Zhang Fabin;Zhang Guangmei;Zhao Xiehui;Yang Yan;Gao Wei(Medical College of Qinghai University,Xining（810001)

机构地区：[1]青海大学医学院,810001

出　　处：《高原医学杂志》2018年第2期15-18,共4页Journal of High Altitude Medicine

基　　金：国家自然科学基金地区科学基金项目(No.81360520)

摘　　要：目的:在高寒环境下复制太阴病脾阳虚证动物模型。方法:大鼠采用大承气汤室温灌胃法造模。造模过程分为两个阶段,共12天。造模结束后观测实验大鼠一般状况及体重,测定血D-木糖浓度等指标的变化。结果:模型组大鼠第13天体质量增长率(2.65±7.78)%、第24天体质量增长率(5.40±4.48)%明显低于正常组(15.00±8.5)%、(13.16±10.59)%(P<0.01),模型组大鼠第13天血D-木糖浓度(0.19±0.01)mmol/L、第24天血D-木糖浓度(0.22±0.01)mmol/L低于正常组(0.31±0.05)mmol/L、(0.30±0.01)mmol/L(P<0.01)。结论:造模12天后,造模大鼠的表现符合中医太阴病(脾阳虚证)的病理变化,说明模型复制成功。This exploration is to reconstruct the animal model of Taiyin disease（syndrome of deficien-cy of spleen yang） in alpine environment. Methods. A rat model was established by using the gavages method of Dachengqi Decoction at a moderate room temperature. The modeling process is divided into two phases with a total of 12 days. After modeling, the general condition and body weight of the rats were observed and the changes of blood D--xylose concentration were measured.Results.The body weight growth rate of the model rats was（2.65± 7.78） % on the 13th day and（5.4±04.48） % on the 24th day , which was significantly lower than that of the nor- mal groupF（15.00±8.5）% and（13.16±10.59）%] （P〈0.01）. The concentration of D-xylose in the rats on the 13th day was （0.19±0.01）mmol/L and （0.22±0.01）mmol/L on the 24th day which was lower than that in the normal group m（0.31±0.05）mmol/L and（0.30±0.01）mmol/L] （P〈0.01）. Conclusion：Twelve days after model- ing, the performance of the model rats was consistent with the pathological changes of TCM Taiyin disease （syn- drome of deficiency of spleen yang）, which indicated that the model was successfully replicated.

关 键 词：高寒地区 太阴病 脾阳虚证 动物模型 

分 类 号：R-33[医药卫生] R22