普瑞巴林对CCI模型大鼠脊髓GFAP和SP表达的影响  

EFFECT OF PREGABALIN ON THE EXPRESSION OF GFAP AND SUBSTANCE P IN THE CCI RAT MODEL

作　　者：张嘉航[1] 韩立伟[1] 曲静波[1] 周华成[2] 贾雅蕊[3] 刘金锋[1] ZHANG Jia-Hang;HAN Li-Wei;QU Jing-Bo;ZHOU Hna-Cheng;JIA Ya-Rui;LIU Jin-Feng(Department of Pain,The Second Affiliated Hospital of Harbin Medical University;Hei Long Jiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine,Harbin 150086,Chin;2Department of Pain,The Fourth Affiliated Hospital of Harbin Medical University,Harbin 150086,Chin;3Department of Anesthesiology,The Heze municipal hospital,Heze 274000,China)

机构地区：[1]哈尔滨医科大学附属第二医院疼痛科黑龙江麻醉与危重病重点实验室,哈尔滨150086 [2]哈尔滨医科大学附属第四医院疼痛科,哈尔滨150086 [3]菏泽市立医院麻醉科,菏泽274000

出　　处：《中国疼痛医学杂志》2018年第11期830-835,共6页Chinese Journal of Pain Medicine

基　　金：黑龙江省教育厅科学技术研究项目(12541562)

摘　　要：目的:观察普瑞巴林(pregabalin, PGB)对坐骨神经慢性压迫损伤(chronic constriction injury,CCI)模型大鼠的脊髓胶质纤维酸性蛋白(glial fibrillary acidic protein, GFAP)和P物质(substance P, SP)表达的影响。方法:SD大鼠随机分为3组(n=10),假手术组(S组)、CCI模型组(C组)、PGB组(P组),于术后第7天开始,P组每天一次PGB 60 mg/kg灌胃,S组和C组均给予同容积生理盐水。测定术前及术后第1、3、5、7、9、11、14 d大鼠机械刺激缩足反射阈值(paw withdrawal mechanical threshold,PWMT)和热刺激缩足反射潜伏期值(paw withdrawal thermal latency, PWTL)。免疫组化法测定大鼠脊髓背角内GFAP和P物质表达情况。结果:与S组相比,C组术后PWMT和PWTL明显降低,术后第7 d最为显著,GFAP及P物质表达明显上调,差异有统计学意义(P <0.05);与C组相比,P组治疗后各时间点PWMT和PWTL显著升高,GFAP和SP表达明显下降,差异显著(P <0.05)。结论:普瑞巴林能够抑制CCI模型大鼠脊髓背角GFAP和SP表达,减轻CCI模型大鼠痛觉过敏。Objective： To investigate the effects of pregabalin（PGB） on the expression of glial fibrillary acidic protein（GFAP） and substance P in rats with chronic constriction injury（CCI） model. Methods： SD rats were randomly divided into three groups（n = 10）, sham operation group（S）, CCI group（C） and pregabalin treatment group（P）. Rats in Group P were given PGB 60 mg/Kg once a day by intragastric infusion from the seventh day after the operation, while rats in Group S and Group C were given equal volume of saline. Paw withdrawal mechanical threshold（PWMT） and paw withdrawal thermal latency（PWTL） were measured before operation and at the day 1, 3, 5, 7, 9, 11, 14 after operation. The expression of GFAP and P substance was detected by immunohistochemical method. Results： Compared with Group S, PWMT and PWTL were significantly decreased in Group C, especially on the 7 th day after operation. The expression of GFAP and substance P was obviously upregulated（P〈 0.05）. Compared with Group C, PWTL and PWMT in Group P were increased significantly and the expression of GFAP and substance P were markedly downregulated（P〈 0.05）. Conclusion： Pregabalin can inhibit the expression of GFAP and substance P in CCI model rats, which may contribute to alleviation of neuropathic pain.

关 键 词：普瑞巴林 神经病理性疼痛 脊髓胶质纤维酸性蛋白 P物质 

分 类 号：R741[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]