靛玉红缓解三硝基苯磺酸致大鼠溃疡性结肠炎及其机制研究  

Effects of indirubin on rats withulcerative colitis induced by TNBS and its mechanism

作　　者：李楠[1] 柳越冬[2] 王长洪[3] 崔世超[1] 张晓明[1] LI Nan;LIU Yue-dong;WANG Chang-hong;CUI Shi-chao;ZHANG Xiao-ming(Graduate School,Liaoning University of Chinese Medicine,Shenyang 110032,China;the Fourth Clinical College of Liaoning University of Chinese Medicine,Shenyang 110101,China;Dept of Traditional Chinese Medicine,the General Hospital of Shenyang Military Region,Shenyang 110016,China)

机构地区：[1]辽宁中医药大学研究生院,辽宁沈阳110032 [2]辽宁中医药大学第四临床学院,辽宁沈阳110101 [3]沈阳军区总医院中医科,辽宁沈阳110016

出　　处：《中国药理学通报》2018年第12期1689-1692,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81072819)

摘　　要：目的观察靛玉红对三硝基苯磺酸诱导的大鼠溃疡性结肠炎(ulcerative colitis,UC)的治疗作用,并探讨其可能机制。方法 48只清洁级SD大鼠,♂,体质量(200±20)g,随机分为4组,分别为正常组、模型组、靛玉红组、柳氮磺吡啶组。造模并治疗7 d后,进行疾病活动指数(disease activity index, DAI)评分,观察结肠HE染色病理切片,检测转化生长因子β1(transforming growth factorβ1,TGF-β1)水平,以及肠三叶因子(intestinal trefoil factor, ITF)mRNA表达。结果与模型组比较,靛玉红组DAI评分降低,结肠HE染色病理显示炎症细胞明显减少,TGF-β1水平降低,ITF mRNA表达升高,差异均有显著性(P<0.05)。结论靛玉红可以明显改善UC模型大鼠症状、结肠病理形态,通过降低TGF-β1水平,上调ITF mRNA表达,从而达到抑制炎症的效果。Aim To observe the effects of indirubin on rats with ulcerative colitis（UC） and explore its possible mechanism. Methods Forty-eight SD rats, body weighted（200±20）g were randomly divided into four groups： control group, model group, indirubin group and SASP group. After seven days′treatment, DAI score was recorded,the colonic HE staining pathological smear, level of TGF-β1 and the expression of ITF mRNA in colonic mucosa of ulcerative colitis of rats were determined. Results Compared with model group, DAI index in indirubin group showed a gradual decreasing trend, and pathological morphology was significantly improved. The level of TGF-β1 decreased and the expression of ITF mRNA increased with significant statistical difference（ P 〈0.05）. Conclusions Indirubin could significantly improve the symptoms and pathological morphology in UC rats by decreasing TGF-β1 level and increasing ITF mRNA expression to reach the effect of inhibiting inflammation.

关 键 词：靛玉红 溃疡性结肠炎 转化生长因子Β1 肠三叶因子 三硝基苯磺酸 炎性肠病 

分 类 号：R-332[医药卫生] R284.1