脑心通胶囊对小鼠后下肢急性缺血模型抗炎作用研究  

Effect of Naoxintong capsule on inflammation in hind limb ischemia injury in mice

作　　者：张璐莎 陈璐[1] 李春晓[1] 尤星宇 房志锐 宋敏[1] Joel Wake Coffie 张丽媛 王虹[1] ZHANG Lu-sha;CHEN Lu;LI Chun-xiao;YOU Xing-yu;FANG Zhi-rui;SONG Min;Joel Wake Coffie;ZHANG Li-yuan;WANG Hong(Tianjin University of Traditional Chinese Medicine,Tianjin State Key Lab of Modern Chinese Medicine-the Ministry of Education to Build National Key Experimental Cultivation Base,Tianjin State Key Lab of Chinese Pharmacology,Tianjin State Key Lab of Prescription Medicine of Ministry of Education,Tianjin 301617,China)

机构地区：[1]天津中医药大学天津市现代中药重点实验室-省部共建国家重点实验室培育基地天津市中药药理学重点实验室方剂学教育部重点实验室,天津301617

出　　处：《中国药理学通报》2018年第12期1767-1773,共7页Chinese Pharmacological Bulletin

基　　金：国家国际科技合作专项(No 2015DFA30430);天津市自然科学基金重点项目(No 16JCZDJC36300);长江学者和创新团队发展计划项目(No PCSIRTIRT16R54);国家自然科学基金青年基金项目(No 81603329)

摘　　要：目的脑心通胶囊(Naoxintong capsule, NXT)可以有效改善组织缺血症状,但NXT对缺血组织炎性反应的影响及作用机制尚不明确。方法本研究采用小鼠后下肢缺血模型,研究NXT对缺血组织炎症作用的影响。采用流式细胞术检测给药后3 d,中性粒细胞、巨噬细胞的阳性细胞比例;采用qRT-PCR检测Emr1、IL-1β、TNF-α的表达;采用蛋白芯片探究炎症相关蛋白的变化,并对差异蛋白进行生物信息学分析。结果术后3 d,与模型组相比,NXT组中性粒细胞、巨噬细胞数量明显下调(P<0.01);Emr1 mRNA基因表达明显下调(P<0.01);炎症因子IL-1β、TNF-α表达明显下调(P<0.05,P<0.01);与模型组相比,NXT上调M2a型巨噬细胞诱导剂IL-4,以及标志物CCL17、CCL22的表达;同时下调M1型巨噬细胞标志物CCL5、CXCL9的表达。结论 NXT在一定程度上可以降低外周动脉疾病伴随的炎症反应,为NXT抗炎的分子机制研究提供了潜在的生物标志物。Aim To explore whether Naoxintong capsule （NXT） can reduce the inflammation after hind limb ischemia （HLI）. Methods To investigate the effect of NXT on inflammation after HLI injury in mice, flow cytometry was used to detect the proportion of neutrophils and macrophages on day 3 after surgery. The expression of Emr1 gene was detected by RT-PCR which was an indicator of macrophages, and RT-PCR was used to detect the expression of inflammatory cytokines IL-1β, TNF-α on day 3 after HLI. Proteome Profler TM Array was carried out to screen and identify the differentially expressed proteins, and the corresponding bioinformatic analysis was done. Results Three days after operation, the number of neutrophils and macrophages in NXT group was significantly smaller than that in model group （ P 〈0.01）, and the expression of Emr1 was significantly down-regulated （ P 〈0.01）. The expression of IL-1β （ P 〈0.05）, TNF-α （ P 〈0.01） in NXT group was significantly lower than that of the untreated group. Compared with model group, NXT up-regulated the expression of IL-4, an inducer of M2a macrophages, as well as CCL17 and CCL22, markers of M2a macrophages, and down-regulated the expression of CCL5, CXCL9, markers of M1 macrophages. Conclusions NXT may reduce the inflammatory response associated with peripheral arterial disease to a certain extent, and provide potential biological markers to a molecular mechanism for anti-inflammatory effect.

关 键 词：脑心通胶囊 后下肢缺血模型 中性粒细胞 巨噬细胞 趋化因子 炎性因子 

分 类 号：R-332[医药卫生] R282.71