Different distributions of interstitial cells of Cajal and platelet-derived growth factor receptor-α positive cells in colonic smooth muscle cell/interstitial cell of Cajal/plateletderived growth factor receptor-α positive cell syncytium in mice  

作　　者：Chen Lu Xu Huang Hong-Li Lu Shao-Hua Liu Jing-Yu Zang Yu-Jia Li Jie Chen Wen-Xie Xu 

机构地区：[1]Department of Physiology, Shanghai Jiao Tong University School of Medicine [2]Department of Anesthesiology, Ren Ji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine [3]Department of Pediatric Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine

出　　处：《World Journal of Gastroenterology》2018年第44期4989-5004,共16页世界胃肠病学杂志（英文版）

基　　金：Supported by The National Natural Science Foundation of China,No.31671192 and No.31571180;Foundation of Xin Hua Hospital,No.JZPI201708

摘　　要：AIM To investigate the distribution and function of interstitialcells of Cajal(ICCs) and platelet-derived growth factor receptor-α positive(PDGFRα+) cells in the proximal and distal colon.METHODS The comparison of colonic transit in the proximal and distal ends was performed by colonic migrating motor complexes(CMMCs). The tension of the colonic smooth muscle was examined by smooth muscle spontaneous contractile experiments with both ends of the smooth muscle strip tied with a silk thread. Intracellular recordings were used to assess electrical field stimulation(EFS)-induced inhibitory junction potentials(IJP) on the colonic smooth muscle. Western blot analysis was used to examine the expression levels of ICCs and PDGFRα in the colonic smooth muscle.RESULTS Treatment with NG-nitro-L-arginine methyl ester hydrochloride(L-NAME) significantly increased the CMMC frequency and spontaneous contractions, especially in the proximal colon, while treatment with MRS2500 increased only distal CMMC activity and smooth muscle contractions. Both CMMCs and spontaneous contractions were markedly inhibited by NPPB, especially in the proximal colon. Accordingly, CyPPA sharply inhibited the distal contraction of both CMMCs and spontaneous contractions. Additionally, the amplitude of stimulationinduced nitric oxide(NO)/ICC-dependent slow IJPs(sIJPs) by intracellular recordings from the smooth muscles in the proximal colon was larger than that in the distal colon, while the amplitude of electric field stimulationinduced purinergic/PDGFRα-dependent fast IJPs(fIJPs) in the distal colon was larger than that in the proximal colon. Consistently, protein expression levels of c-Kit and anoctamin-1(ANO1) in the proximal colon were much higher, while protein expression levels of PDGFRα and small conductance calcium-activated potassium channel 3(SK3) in the distal colon were much higher.CONCLUSION The ICCs are mainly distributed in the proximal colon and there are more PDGFRα+ cells are in the distal colon, which generates a pressure gradAIM To investigate the distribution and function of interstitial cells of Cajal （ICCs） and platelet-derived growth factor receptor-α positive （PDGFRα＋） cells in the proximal and distal colon.METHODSThe comparison of colonic transit in the proximal and distal ends was performed by colonic migrating motor complexes （CMMCs）. The tension of the colonic smooth muscle was examined by smooth muscle spontaneous contractile experiments with both ends of the smooth muscle strip tied with a silk thread. Intracellular recordings were used to assess electrical field stimulation （EFS）-induced inhibitory junction potentials （IJP） on the colonic smooth muscle. Western blot analysis was used to exa-mine the expression levels of ICCs and PDGFRα in the colonic smooth muscle.RESULTSTreatment with NG-nitro-L-arginine methyl ester hydro-chloride （L-NAME） significantly increased the CMMC frequency and spontaneous contractions, especially in the proximal colon, while treatment with MRS2500 in-creased only distal CMMC activity and smooth muscle contractions. Both CMMCs and spontaneous contractions were markedly inhibited by NPPB, especially in the pro-ximal colon. Accordingly, CyPPA sharply inhibited the distal contraction of both CMMCs and spontaneous contractions. Additionally, the amplitude of stimulation-induced nitric oxide （NO）/ICC-dependent slow IJPs （sIJPs） by intracellular recordings from the smooth muscles in the proximal colon was larger than that in the distal colon, while the amplitude of electric field stimulation-induced purinergic/PDGFRα-dependent fast IJPs （fIJPs） in the distal colon was larger than that in the proximal colon. Consistently, protein expression levels of c-Kit and anoctamin-1 （ANO1） in the proximal colon were much higher, while protein expression levels of PDGFRα and small conductance calcium-activated potassium channel 3 （SK3） in the distal colon were much higher.CONCLUSIONThe ICCs are mainly distributed in the proximal colon and there are more PDGFRα＋

关 键 词：Interstitial cells of Cajal Platelet-derived growth factor receptor-α positive cells Smooth muscle cell/interstitial cell of Cajal/platelet-derived growth factor receptor-α positive cell syncytium Nitric oxide PURINE 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]