藏红花素Ⅰ的分离制备及抗氧化与稳定性研究  

Study on Separation and Preparation of Crocin Ⅰ with Its Antioxidation and Stability

作　　者：陈哲 赵庆生[2] 秦雯[1] 赵兵[2] 葛思琪 赵强 查圣华[2] CHEN Zhe;ZHAO Qing-sheng;QIN Wen;ZHAO Bing;GE Si-qi;ZHAO Qiang;ZHA Sheng-hua(Beijing City University,Beijing 100094,China;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;Nanyang Tairui Biotechnology Co.,Ltd.,Nanyang 473412,Henan,China)

机构地区：[1]北京城市学院,北京100094 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [3]南阳泰瑞生物科技股份有限公司,河南南阳473412

出　　处：《食品研究与开发》2018年第24期63-70,共8页Food Research and Development

摘　　要：建立硅胶柱层析法分离制备栀子黄色素中藏红花素Ⅰ的方法,并对所得产品进行抗氧化性与稳定性研究。建立了乙酸乙酯-甲醇-水(体积比为100∶20∶15至100∶25∶15)的梯度洗脱体系,以及层析柱硅胶与栀子黄色素的质量比为350∶1的上样量比例,并制备出纯度为98.39%的藏红花素Ⅰ单体样品,其得率为15.58%,回收率为50.42%。抗氧化与稳定性研究结果表明,藏红花素Ⅰ具有优良的抗氧化活性;藏红花素Ⅰ在高温、强光照射、强酸强碱环境中均会产生损失。含藏红花素Ⅰ成分的产品应贮存于低温、避光、pH值为6的环境中,且与适量抗坏血酸联用可以提高其稳定性。To establish a method tor the separation and preparation of crocinⅠ from gardenia yellow pigment by silica gel column chromatography, and to study the antioxidation and stability of the obtained product. Established a gradient elution system was ethyl acetate-methanol-water （100 ： 20 ： 15 to 100 ： 25 ： 15 by volume） and the optimal proportion of the loading amount was the mass ratio of silica gel to gardenia yellow pigment with 350 ： 1, and a 98.39 % purity sample of cretin Ⅰ was prepared with a yield of 15.58 % and a recovery rate of 50.42 %. The results of antioxidant and stability studies showed that crocin Ⅰhad excellent antioxidant activity, and cretin Ⅰ was lost in high temperature, strong light, strong acid and alkali environment. The products containing crocin Ⅰ should be stored in an environment with low-temperature, light-proof, pH 6, and can be used in combination with an appropriate amount of ascorbic acid to improve its stability.

关 键 词：栀子黄色素 藏红花素Ⅰ 硅胶柱层析 稳定性 体外抗氧化 

分 类 号：R91[医药卫生—药学]