应用四种体外消化方法比较研究场地土壤中重金属的生物可给性及其人体健康风险  

Bioaccessibility and human health risk assessement of heavy metals in soils by using four in vitro digestion methods

作　　者：陈廷廷 侯艳伟 蔡超[2] 池海峰 张又弛[2] 廖用开 CHEN Tingting;HOU Yanwei;CAI Chao;CHI Haifeng;ZHANG Youchi;LIAO Yongkai(Department of Environmental Science and Engineering,College of Chemical Engineering,Huaqiao University,Xiamen,361021,China;State Key Laboratory of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen,361021,China)

机构地区：[1]华侨大学化工学院环境科学与工程系,厦门361021 [2]中国科学院城市环境与健康重点实验室中国科学院城市环境研究所,厦门361021

出　　处：《环境化学》2018年第11期2342-2350,共9页Environmental Chemistry

基　　金：国家自然科学基金青年基金(41501525);福建省环保科技计划项目(Y6F0771B70)资助~~

摘　　要：本研究采用PBET(physiologically based extraction test)、DIN(Deutsches Institut für Normung)、IVG(Invitro Gastrointestinal)和UBM(Unified BARGE Method)等4种体外消化方法,比较8个工矿废弃场地土壤中6种典型重金属的生物可给性并进行健康风险评估分析.结果显示,在4种方法的胃阶段,土壤Co、Ni、Cu、Zn、As和Cd的生物可给性范围分别为1.57%—88.07%、6.69%—129.78%、8.68%—122.62%、18.55%—128.75%、4.17%—61. 14%和32. 22%—118. 44%;小肠阶段分别为0—43. 49%、3. 12%—61. 94%、4. 75%—123. 91%、1.48%—99.02%、5.93%—39.70%和8.48%—67.83%.总体而言,采集的场地土壤中多数重金属生物可给性在各方法的胃阶段均显著高于小肠阶段.土壤重金属在胃阶段的生物可给性分析显示,Co、Ni、Cu和As在UBM和IVG方法中较高,Zn在DIN方法中最高,Cd在UBM和DIN方法中高于其他方法.土壤重金属在小肠阶段的生物可给性分析显示,Co和Ni在UBM方法中最高,Cu在DIN和UBM方法中高于PBET和IVG方法; Zn生物可给性的顺序为DIN>PBET>IVG>UBM; As和Cd在IVG方法中均较高.与基于总量的重金属健康风险评估相比,采用生物可给性为评估参数,对8个场地土壤进行的评估分析显示风险水平显著降低,本研究中的场地土壤若采用基于总量的评估方法可能会高估重金属污染的风险.Four in vitro digestion methods,namely physiologically based extraction test（ PBET）,Deutsches Institut für Normung（ DIN）,In-Vitro gastrointestinal（ IVG） and Unified BARGE Method（ UBM） were used to estimate the bioaccessibility and human health risks of heavy metals in soils from eight abandoned mines and industries. The results showed that,among the four vitro methods,the ranges of bioaccessibilities of Co,Ni,Cu,Zn,As and Cd were 1. 57%—88. 07%,6. 69%—129.78%,8. 68%—122. 62%,18. 55%—128. 75%,4. 17%—61. 14% and 32. 22%—118. 44% in the gastric phase,respectively. In the intestinal phase,the ranges of bioaccessibilities of Co,Ni,Cu,Zn,As and Cd were 0—43. 49%,3. 12%—61. 94%,4. 75%—123. 91%,1. 48%—99. 02%,5.93%—39.70% and 8.48%—67.83%,respectively. In general,the bioavailability of most heavy metals in the gastric phase was significantly higher than that in the intestinal phase. In the gastric phase,the bioaccessibilities of Co,Ni,Cu and As by the UBM and IVG methods were higher than by the other methods,and Zn bioaccessibility by DIN method was the highest among the four vitro methods. But Cd bioaccessibility by DIN and UBM methods was higher than by the other methods. In the intestinal phase, UBM method exhibited the highest bioaccessibilities of Co and Ni. The bioaccessibilities of Cu by DIN and UBM methods were higher than by the other methods. The order of Zn bioaccessibility by the different methods was DIN〉PBET〉IVG〉UBM. In the intestinal phase,IVG method exhibited the highest bioaccessibility of As. And Cd bioaccessibilities by PBET and IVG methods were higher than by DIN and UBM methods. Compared with the health risk assessment based on heavy metal total concentrations,the risk level evaluated by the bioaccessibility decreased significantly. So in this study, the health risk assessement based on total heavy metals may overestimate their risk in soils.

关 键 词：土壤 体外消化 重金属 生物可给性 人体健康风险评估 

分 类 号：X53[环境科学与工程—环境工程]