藤三七总黄酮的超声波辅助提取及其抗氧化性研究  

Ultrasonic-assisted Extraction of Total Flavonoids from Boussingaultia gracilis and its Antioxidant Activity

作　　者：杨申明 王振吉 戴玥 YANG Shen-ming;WANG Zhen-ji;DAI Yue(Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong 675000,China)

机构地区：[1]楚雄师范学院化学与生命科学学院,云南楚雄675000

出　　处：《保鲜与加工》2018年第5期65-71,共7页Storage and Process

基　　金：国家自然科学基金青年项目(31300370);云南省应用基础研究青年项目(2014FD052);云南省高校科技创新团队支持计划项目(IRTSTYN);云南省省级重点学科建设基金项目(05YJJSXK03);楚雄师范学院教改项目(1510)

摘　　要：以藤三七为原料,采用超声波辅助提取总黄酮,利用正交试验优化其提取工艺,并从清除羟基自由基(·OH)、1,1-苯基-2-苦基肼自由基(DPPH·)和超氧阴离子自由基(O2-·)的能力3个角度评价其抗氧化性。结果表明,超声波辅助提取藤三七总黄酮的最佳提取工艺为:乙醇体积分数80%,液料比40∶1(m L/g),超声时间60 min,超声温度40℃。在该条件下,总黄酮平均提取率为18.51%。超声波辅助提取的总黄酮具有较强的抗氧化性,对·OH、DPPH·、O2-·清除作用明显,且其质量浓度与抗氧化活性呈量效关系,是一种良好的天然抗氧化剂。The total flavonoids from Boussingaultia gracilis was extracted by ultrasonic-assisted extraction, orthogonal experiment was used to optimize the extraction process, and the antioxidant activities were evaluated in three systems： hydroxyl radical（·OH）, 1,1-diphenyl-2-picrylhydrazxyl radical（DPPH·） and superoxide anion free radical scavenging capacity. The optimal extraction conditions were obtained as follows： the volume fraction of ethanol was80%, liquid-solid ratio was 40∶1（mL/g）, ultrasonic time was 60 min, and ultrasonic temperature was 40 ℃. Under these conditions, the average extraction rate of total flavonoids was 18.51%. Total flavonoids extracted by ultrasonic method had obvious scavenging activity against hydroxyl radical, DPPH radical and superoxide anion free radical in a concentration-dependent manner, which indicated that Boussingaultia gracilis total flavonoids were good natural antioxidants.

关 键 词：藤三七 超声波辅助提取 总黄酮 抗氧化性 

分 类 号：TQ914.1[化学工程]