我国部分新型布尼亚病毒分离株全基因组序列分析  

Sequence analysis of the complete genome sequence of Severe fever with thrombocytopenia syndrome virus isolated in China

作　　者：刘秀兰[1] 姚学君 张雪峰[2] 管书慧[1] 姜仁杰[1] LIU Xiu-lan;YAO Xue-jun;ZHANG Xue-feng;GUAN Shu-hui;JIANG Ren-jie(Yancheng Municipal Center for Disease Prevention and Control,Jiangsu Yancheng 224100,China)

机构地区：[1]盐城市疾病预防控制中心,江苏盐城224100 [2]江苏省疾病预防控制中心

出　　处：《江苏预防医学》2018年第6期623-625,648,共4页Jiangsu Journal of Preventive Medicine

基　　金：江苏省重大传染病防控科技示范项目(BE2015714);江苏省流行病学重点学科(ZDXK A2016008)

摘　　要：目的 分析我国新型布尼亚病毒分离株全基因组序列，了解遗传进化特点。 方法 利用DNA STAR和MEGA 6.0软件，对GenBank数据库收录的我国75株分离株全基因组序列进行分析，构建系统进化树，并对毒株核苷酸和氨基酸突变位点进行统计。 结果 75株新型布尼亚病毒分离株流行优势株为基因型C；分离株核苷酸碱基突变总数合计5 938 bp，主要为碱基转换（占79.86%）；氨基酸间突变总数合计3 304 aa。 结论 我国新型布尼亚病毒全基因组序列系统进化树具有相似的拓补结构。Objective To analyze the complete genome sequence of severe fever with thrombocytopenia syndrome virus （SFTSV）isolated in China; to understand molecular epidemiological characteristics of SFTSV. Methods DNA STAR and MEGA 6.0 software were used for complete genome sequence analysis, phylogenetic analysis and the nucleotide and amino acid mutation sites of 75 isolated virus in Chinathrough retrieving the GenBank nucleotide database. Results Genotype C was the domirant strain in recent years; the base sequence mutation were amounted to 5 938 bp, and the major type was base transition; the amino acid substitutions amounted to 3 304 aa. Conclusion The phylogenetic tree of the complete genome sequence of SFTSV isolated in China have similar topological structures.

关 键 词：新型布尼亚病毒 全基因组 序列分析 GENBANK数据库 

分 类 号：R373[医药卫生—病原生物学]