miR-202、AFP及DKK1在原发性肝癌患者血清中的表达及其意义  

Expression and significance of miR-202, AFP and DKK1 in serum of patients with primary hepatic carcinoma

作　　者：韩悦[1] 魏殿军[1] HAN Yue;WEI Dian-jun(Department of Clinical Laboratory,The Second Hospital,Tianjin Medical University,Tianjin 300211,China)

机构地区：[1]天津医科大学第二医院检验科,天津300211

出　　处：《天津医科大学学报》2018年第6期517-520,共4页Journal of Tianjin Medical University

基　　金：天津市卫计委科技基金资助项目(2015KZ101)

摘　　要：目的:研究微小RNA-202(miR-202)、甲胎蛋白(AFP)、分泌性蛋白Dikkopf-1(DKK1)在原发性肝癌(PHC)患者血清中的表达水平及临床价值。方法:选择2013年6月-2014年12月我院肝病科收治的PHC患者(PHC组)43例、良性肝病患者(良性肝病组)37例作为研究对象,选择同期健康体检人员51例作为健康对照组。检测3组受试对象血清中miR-202、AFP及DKK1的表达水平,运用受试者工作特征曲线(ROC曲线)分析miR-202、AFP、DKK1联合检测诊断PHC的诊断效能。结果:(1)PHC患者血清中miR-202水平低于良性肝病组和健康对照组,有显著性差异(P<0.05);AFP和DKK1水平均高于良性肝病组和健康对照组,差异具有统计学意义(P<0.05)。(2)联合检测诊断PHC的效能高于miR-202、AFP及DKK1单独检测。(3)miR-202与AFP呈负相关(r=-0.314,P<0.05);miR-202与DKK1呈负相关(r=-0.319,P<0.05);AFP与DKK1呈正相关(r=0.556,P<0.05)。结论:原发性肝癌患者miR-202水平表达水平降低,AFP和DKK1表达水平升高,三者联合检测有助于原发性肝癌的临床诊治。Objective： To study the expression level and clinical value of tiny RNA-202 （miR-202）, alpha fetoprotein （AFP） and secretory protein Dikkopf-1 （DKK1） in serum of patients with primary hepatic carcinoma （PHC）. Methods ： From June 2013 to December 2014, 43 patients with PHC （PHC group） and 37 patients with benign liver disease （benign liver disease group） were selected as the research subjects. Fifty-one healthy persons during the same period were selected as healthy control group. The levels of serum miR-202, AFP and DKK1 in three groups were detected. Receiver operating characteristic curve （ROC curve） was used to analyze the diagnostic efficiency of miR-202, AFP and DKK1 in the diagnosis of primary hepatic carcinoma. Results：The serum level of miR-202 in PHC group was lower than that in benign liver disease group and healthy control group （P〈 0.05）. AFP and DKK1 levels were all higher than those in benign liver disease group and healthy control group, and the difference was statistically significant （P〈 0.05）. The efficacy of joint detection in the diagnosis of PHC was higher than that of miR-202, AFP and DKKI.MiR-202 was negatively correlated with AFP （r=-0.314, P〈0.05）; miR-202 was negatively correlated with DKK1 （r=-0.319, P〈 0.05）; AFP was positively correlated with DKK1 （r=0.556, P〈0.05）. Conclusion：The expression levels of miR-202 and AFP and DKK1 could increase in patients with primary hepatic cancer. The combined test of the three may be useful to the clinical diagnosis and treatment of primary hepatic cancer.

关 键 词：miRNA-202 甲胎蛋白 分泌性蛋白Dikkopf-1 原发性肝癌 

分 类 号：R735.7[医药卫生—肿瘤]