Box-Behnken响应面法优化辣木叶蛋白超声提取工艺研究  

Optimization of ultrasound extraction process for protein from leaves of Moringa oleifera by Box-Behnken response surface methodology

作　　者：郝东宇 席兴军[2] 初侨[2] 赵新颖[3] 王尉[3] 张维冰[1] 兰韬[2] Hao Dongyu;Xi Xingjun;Chu Qiao;Zhao Xinying;Wang Wei;Zhang Weibing;Lan Tao(Qiqihar University,Qiqihar 1GlOOG,China;China Institute of Standardization,Beijing 100191,China;Beijing Physical and Chemical Analysis and Testing Center,Beijing 100089,China)

机构地区：[1]齐齐哈尔大学,齐齐哈尔161006 [2]中国标准化研究院,北京100191 [3]北京市理化分析测试中心,北京100089

出　　处：《分析仪器》2018年第6期128-136,共9页Analytical Instrumentation

基　　金：国家重点研发计划(2017YFF0207800);中央级公益性科研院所基本科研业务费(562017Y-5284)资助

摘　　要：为了更高效利用辣木叶中的蛋白质,以干燥的辣木叶为原料,通过超声提取法从辣木叶样品中提取蛋白质。通过单因素试验的方法分别考察了溶剂pH值、料液比、提取温度、超声功率和提取时间5个因素对辣木叶中蛋白质提取效率的影响。通过Design Expert 8.0.6软件中的Box-Behnken模型对实验结果的数据拟合分析可知,pH值、提取时间、超声功率、料液比和提取温度对蛋白质提取率的影响依次降低;但是pH值和提取时间对辣木叶中蛋白提取率的影响最为显著;分析得出最佳的提取条件,即pH值10.0、料液比1∶122 (g/mL)、超声功率51W、提取温度50℃、提取时间53min,辣木叶中蛋白质的回收率为42.3%。在此实验条件下,对全国随机取样的76种辣木叶相关产品中蛋白质进行提取比较,结果显示90%以上辣木叶产品的蛋白质含量都在20%以上,说明辣木叶中的蛋白质含量非常高,可以作为植物蛋白的替代来源,为现有辣木相关产品的深度开发和利用提供了应用技术支持。Five influence factors on the ultrasound extraction process, including the pH value of sol vent, the ratio of material to liquid, the extraction temperature, the ultrasonic power and the extraction time were investigated by single factor test. The influence of pH value, extraction time, ultrasonic power, material to liquid ratio and extraction temperature on protein extraction rate decreased in turn. The influence of pH value and extraction time were more significant. The optimum extraction conditions were as follows：pH value 10, material to liquid ratio 1：122 （g/mL）, ultrasonic power 51W, extraction temperature 50℃, extraction time 53 min. The recovery of protein was 42.3%. In this experiment, the protein in 76 products of Moringa oleifera grown in China were extracted and compared. The results were good.

关 键 词：辣木叶 蛋白质 超声提取 蛋白质提取率 Box-Behnken响应面法 

分 类 号：TQ936[化学工程]