酮体抑制大鼠海马神经元酸敏感通道的开放  

Ketone bodies inhibits the opening of acid-sensing ion channels in hippocampal neurons of rats

作　　者：朱飞[1] 徐秦岚 郭安臣[1] 王群[1] Zhu Fei;Xu Qinlan;C-uo Anchen(Department of Neurology,Beijing Tiantan Hospital,Capital Medical University,China National Clinical Research Center for Neurological Diseases,Beijing Institute for Brain Disorders,Beijing 100050)

机构地区：[1]首都医科大学附属北京天坛医院神经病学中心北京脑重大疾病研究国家神经系统疾病临床医学研究中心,北京100050

出　　处：《卒中与神经疾病》2018年第5期493-496,509,共5页Stroke and Nervous Diseases

基　　金：癫痫病临床医学研究北京市重点实验室2016开放课题(2016DXBL02);北京市优秀人才培养资助-青年骨干个人项目(2016000021469G217);首都医科大学2017年度校自然基金(PYZ2017132)

摘　　要：目的探讨酮体对酸敏感通道的影响。方法原代培养大鼠海马神经元,利用膜片钳技术检测乙酰乙酸、β羟丁酸和丙酮三种酮体对海马神经元酸敏感通道开放状态的影响。结果在pH 6.0条件下细胞外液中分别加入乙酰乙酸、β羟丁酸和丙酮,酸敏感通道电流均明显减弱。三种酮体均可显著抑制海马神经元表面酸敏感通道的开放,抑制率分别为92、47和77%。结论酮体可以显著抑制酸敏感通道的开放。生酮饮食治疗难治性癫痫有可能是通过酮体抑制酸敏感通道来实现。Objective To study the effects of ketone bodies on acid-sensing ion channels.Methods In this study the primary hippocampal neurons of rats were selected as the objectives.The effects of acetoacetic acid,β-hydroxybutyric acid and acetone on the open state of acid-sensing ion channels of hippocampal neurons were investigated by patch-clamp technique.Results Under the condition of pH 6.0 the addition of acetoacetic acid,β-hydroxybutyric acid and acetone in the extracellular solution obviously weakened the currents of acidsensing ion channels.All of three ketone bodies could significantly inhibit the opening of the acid-sensing channels on the surface of hippocampal neurons.The inhibition rates were 92%,47% and 77% respectively.Conclusion Ketone bodies could significantly inhibit the opening of acid-sensing ion channels.Ketogenic diet might achieve the treatment of intractable epilepsy through the ketone body inhibition of acid-sensitive ion channels.

关 键 词：酮体 生酮饮食 酸敏感通道 膜片钳 神经元 

分 类 号：R742[医药卫生—神经病学与精神病学]