导入nodVW基因对费氏中华根瘤菌HH103共生固氮的影响  

Effect of nodVW Genes on Symbiotic Nitrogen Fixation of Sinorhizobium fredii HH103

作　　者：安琦[1,2] 吴皓琼[1,2] 殷博[1,2] 原韬[1,2] 袁明[3] 曹亚彬[1,2] 牛彦波[1,2] 马银鹏[1] AN Qi;WU Hao-qiorlg;YIN Bo;YUAN Zao;YUAN Ming;CAO Ya-bin;NIU Yan-bo;MA Yin-peng(Institute of Microbiology,Heilongjiang Academy of Scienees,Harbin 150010,China;Institute of Advanced Technology,Heilongjiang Academy of Sciences,Harbin 150020,China;Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161006,China)

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]黑龙江省科学院高技术研究院,黑龙江哈尔滨150020 [3]黑龙江省农业科学院齐齐哈尔分院,黑龙江齐齐哈尔161006

出　　处：《大豆科学》2018年第6期871-875,共5页Soybean Science

基　　金：国家现代农业产业技术体系(CARS-004-CES04);国家现代农业产业技术体系子课题(CARS-210301);黑龙江省科学院学部委员指导专项(XB2015SW01);黑龙江省科学院青年创新基金面上项目(CXMS2017SW02);黑龙江省院所基本应用技术研究专项(ZNBZ2016SW01)

摘　　要：本研究以慢生型根瘤菌(Bradyrhizobium japonicum) USDA110为出发菌株,扩增慢生型根瘤菌中双组份调控基因nodVW,并将其导入快生型根瘤菌(Sinorhizobium fredii) HH103及HH103 nod D1失活突变株中,研究重组根瘤菌菌株对大豆嫩丰16共生固氮的影响。结果表明:慢生型大豆根瘤菌双组份调控系统NodVW的导入使大豆植株的株高和鲜重显著降低,大豆植株根部结瘤数量明显减少,NodVW的导入阻碍了快生型根瘤菌的结瘤固氮途径,从而抑制了快生型根瘤菌与大豆结瘤。In this study, Bradyrhizobium japonicum USDA110 was used as the starting strain. The two component regulatory genes nodVW from Bradyrhizobium japonicum USDA110 were amplified and introduced to Sinorhizobium fredii HHI03 and HHI03 nodD1 mutant strains in order to study the effect of nodVW on symbiotic nitrogen fixation of the recombinant strains to soybean genotype NF-16. The results showed that the introduction of the two-component control system NodVW from Bradyrhi- zobium japonicum USDA110 exert negative effect on soybean plants, which significantly reduced the height and fresh weight of soybean plants. In the meanwhile, the number of nodules at soybean roots decreased significantly. The results indicated that the introduction of NodVW hindered the pathway and inhibits the nodulation and nitrogen fixation of Sinorhizobium fiedii HH103 accordingly.

关 键 词：双组份调控系统 nodVW 重组根瘤菌 结瘤固氮 

分 类 号：S565.1[农业科学—作物学]