线虫胁迫下大豆肌醇加氧酶活力研究  

Study on Activity of Myo-inositol Oxygenase Under Stress of Nematodes

作　　者：王学敏[1] 王晗[1] 王超[1] 王爽 于佰双[2] 陈井生[2] 王惠[1] 段玉玺[3] WANG Xue-min;WANG Han;WANG Chao;WANG Shuang;YU Bai-shuang;CHEN Jing-sheng;WANG Hui;DUAN Yu-xi(College of Bioscience and Biotechnology,Shenyang Agricultural University,Liaoning 110866,China;Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866 [2]黑龙江省农业科学院,黑龙江哈尔滨1500862 [3]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《大豆科学》2018年第6期928-931,共4页Soybean Science

基　　金：国家自然科学基金重点项目资助(31330063)

摘　　要：为确定大豆胞囊线虫侵染大豆根部后,肌醇加氧酶活力的变化,以感病品种辽豆15和抗病品种灰皮支黑豆、哈尔滨小黑豆和小粒黑豆为试验材料,在人工接种大豆胞囊线虫3号生理小种后,利用肌醇加氧酶(MIOX)酶联免疫试剂盒检测肌醇加氧酶活力的变化情况。结果表明,在接种后的第5天,感病品种辽豆15酶活力达到最大值,约为对照组的1. 61倍。而抗病品种哈尔滨小黑豆、灰皮支黑豆和小粒黑豆分别在接种后的第10天、第15天、第5天肌醇加氧酶活力达到最高,分别是对照组酶活力的1. 46,1. 51,1. 85倍。Inorder to determine the activity of Myo-inositol oxygenase after soybean cyst infected soybean root. A susceptible cultivar Liaodou 15 and the resistant cuhivars Huipizhi heidou, Harbin Xiaoheidou and Xiaoliheidou were used inositol oxyge- nase （MIOX） elisa kit to detect changes of the inositol oxygenase enzyme activity from soybeans roots after SCN3 infection. The results showed that 5 d after inoculation, Liaodoul5 cultivars enzyme activity reached the maximum value, which was a- bout 1.61 times in the control group. And disease-resistant cultivars of Harbin Xiaoheidou and Xiaoliheidou and Huipizhi Hei- dou, in the 10, 15, 5 d after inoculation, inositol oxygenase reached the highest enzyme activity were the control enzyme ac- tivity of 1.46 times, 1.51 times, 1.85 times respectively.

关 键 词：大豆 肌醇加氧酶 大豆胞囊线虫 酶活力 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]