携带杀白细胞素基因金黄色葡萄球菌5种超抗原肠毒素基因型的相关性研究  

Correlation of five superantigen enterotoxins genotypes in Staphylococcus aureus carrying with Panton-valentine leucocidin

作　　者：奚经巧[1] 林腾涛 曾云祥[1] 孔万仲[1] 梁世周 金晓立[1] 毛瑞奔 许建平[1] XI Jing-qiao;LIN Teng-tao;ZENG Yun-xiang;KONG Wan-zhong;LIANG Shi-zhou;JIN Xiao-li;MAO Rui-ben;XU Jian-ping(Wenzhou Hospital of Traditional Chinese Medicine,Wenzhou,Zhejiang 325000,China)

机构地区：[1]温州市中医院检验科 [2]温州医科大学仁济学院,浙江温州325000

出　　处：《中华医院感染学杂志》2018年第22期3361-3364,共4页Chinese Journal of Nosocomiology

基　　金：温州市公益性社会发展(医疗卫生)科技基金资助项目(Y20160500);温州市鹿城区科技计划基金资助项目(S10105)

摘　　要：目的研究金黄色葡萄球菌中杀白细胞素(PVL)、MecA和五种超抗原肠毒素基因型(SEs)及其相关性。方法收集2012年1月-2017年2月间医院临床分离的206株非重复金黄色葡萄球菌,共检测出17株PVL阳性的金黄色葡萄球菌。用PCR法检测17株PVL阳性的金葡菌与随机抽取的32株PVL阴性金葡菌中的五种超抗原肠毒素基因(SEA、SEB、SEC、SED、SEE)。结果 17株PVL阳性的金黄色葡萄球菌中共检出SEA、SEB、SEC、SED、SEE的阳性菌株分别为10、7、7、0、10。32株PVL阴性的金黄色葡萄球菌中共检出SEA、SEB、SEC、SED、SEE的阳性菌株分别为17、2、5、1、11。两组比较,SEB和SEC基因检出率差异有统计学意义(P<0.05);SEA、SED和SEE基因检出率无统计学差异。PVL(+)耐甲氧西林的金黄色葡萄球菌(MRSA)和PVL(+)甲氧西林敏感的金黄色葡萄球菌(MSSA)的超抗原肠毒素基因检出率差别无统计学意义。结论 PVL阳性的金葡菌其携带超抗原肠毒素基因的概率高于PVL阴性的金葡菌,尤其是SEB和SEC基因在PVL+的金黄色葡萄球菌中更多见。OBJECTIVE To study the panton-valentine leucocidin（PVL）,MecA and five superantigen enterotoxins genotypes（SEs）in Staphylococcus aureus strains and analyze their correlation.METHODS A total of 206 strains of non-repetitive S.aureus were isolated from Wenzhou Hospital of Traditional Chinese Medicine from Jan 2012 to Feb 2017,and totally 17 strains were tested positive for PVL.The five SEs（SEA,SEB,SEC,SED,SEE）in the17 strains of PVL-positive S.aureus and 32 strains of PVL-negative S.aureus that were randomly collected were detected by using PCR.RESULTS Of the 17 strains of PVL-positive S.aureus,10 were detected with SEA,7 SEB,7 SEC,7 SED,0 SEE.Of the 32 strains of PVL-negative S.aureus,17 were detected with SEA,2 SEB,5 SEC,1 SED,11 SEE;there were significant differences in the detection rates of SEB and SEC between the two groups（P〈0.05）;there were no significant differences in the detection rates of SEA,SED and SEE.There were no significant differences in the detection rates of the five SEs between the PVL-positive methicillin-resistant S.aureus（MRSA）strains and the PVL-positive methicillin-sensitive S.aureus（MSSA）strains.CONCLUSIONThe PVL-positive S.aureus strains are more likely to be detected with the SEs than the PVL-negative strains;SEB and SEC are more common in the PVL-positive S.aureus strains.

关 键 词：杀白细胞素 耐甲氧西林金黄色葡萄球菌 甲氧西林敏感金黄色葡萄球菌 金黄色葡萄球菌肠毒素 

分 类 号：R378.1[医药卫生—病原生物学]