直肠癌手术前后血清miR-24-3p和FOXQ1水平变化及其与预后的关系研究  

The study on changes of serum MiR-24-3p and FOXQ1 levels in rectal cancer patients before and after operation and their relationships with prognosis

作　　者：杨洲[1] 刚海菊 王波[1] 覃先蓬[1] 贾贵清[1] 周晓刚[1] YANG Zhou;GANG Hai-ju;WANG Bo;QIN Xian-peng;JIA Gui—qing，ZHOU Xiao—gang(Department of Gastrointestinal Surgery,Sichuan Academy of Medical ple＇s Hospital,Chengdu 610072,China;Chengdu Vocational-Technical Institute，Chengdu 610000，China)

机构地区：[1]四川省人民医院胃肠外科,四川成都610072 [2]成都市职业技术学院医护分院,四川成都610000

出　　处：《中国中西医结合消化杂志》2018年第10期823-828,共6页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：四川省卫计委科研立项课题(No:17PJ109)

摘　　要：[目的]研究直肠癌患者手术前后血清miR-24-3p和叉头框蛋白Q1(FOXQ1)水平变化及其与预后的关系。[方法]选择2011年2月~2014年10月在我院行根治性手术切除的64例直肠癌患者(直肠癌组)作为研究对象,同时期体检健康且无肿瘤家族史的50例正常者(正常组)作为对照,检测直肠癌患者手术前后及正常组血清miR-24-3p和FOXQ1水平,分析血清miR-24-3p和FOXQ1水平与直肠癌患者临床病理特征和3年生存率的关系。[结果]直肠癌组手术前和手术后血清miR-24-3p相对表达量均显著低于正常组(P<0.05),血清FOXQ1水平均显著高于正常组(P<0.05),直肠癌患者术后血清miR-24-3p相对表达量显著升高,血清FOXQ1水平显著降低;直肠癌患者术后半年内复发患者血清miR-24-3p相对表达量显著低于未复发患者(P<0.05),血清FOXQ1水平显著高于未复发患者(P<0.05)。血清miR-24-3p表达水平与直肠癌患者TNM分期、浸润程度及淋巴结转移有关(P<0.05),血清FOXQ1水平与直肠癌患者肿瘤大小、TNM分期、浸润程度及淋巴结转移有关(P<0.05);直肠癌组患者血清miR-24-3p和FOXQ1表达水平呈显著负相关关系(r=-0.762,P=0.000);术后血清miR-24-3p升高率>48.15%患者3年生存率显著高于升高率<48.15%患者(P<0.05),血清FOXQ1降低率>33.69%患者3年生存率显著高于降低率<33.69%患者(P<0.05);FOXQ1表达水平是影响直肠癌患者预后的危险因素,而miR-24-3p表达水平是直肠癌患者预后的保护因素。[结论]直肠癌患者术后血清miR-24-3p水平显著升高,FOXQ1水平显著降低,其升高或降低程度与患者远期预后密切相关。[Objective]To study the changes of serum miR-24-3 p and forking box protein Q1(FOXQ1)levels in rectal cancer patients before and after operation and their relationships with prognosis.[Methods]From February 2011 to October 2014,64 cases of rectal cancer patients(rectal cancer group)treated with radical resection in our hospital were selected as subjects.50 normal subjects(normal group)which were tested without family history of tumor were selected as control,the levels of serum miR-24-3 p and FOXQ1 were measured before and after operation in rectal cancer patients,analyzed the relationships between serum miR-24-3 p and FOXQ1 levels with clinicopathological features and 3-year survival rate in rectal cancer patients.[Results]The relative expressions of serum miR-24-3 p in rectal cancer group before operation and after operation were significantly lower than those in normal group(P<0.05),serum FOXQ1 levels weresignificantly higher than those in the normal group(P<0.05),the relative expression of serum miR-24-3 p in rectal cancer patients after operation increased significantly,the level of serum FOXQ1 decreased significantly;the relative expression of serum miR-24-3 p in patients with rectal cancer recurrence in six months after operation was significantly lower than that in patients without recurrence(P<0.05),serum FOXQ1 level was significantly higher than that of non recurrence patients(P<0.05).The expression of miR-24-3 p in serum was correlated with TNM stage,degree of invasion and lymph node metastasis(P<0.05),serum FOXQ1 level was correlated with tumor size,TNM stage,infiltration degree and lymph node metastasis in rectal cancer(P<0.05);there was a significant negative correlation between serum miR-24-3 p and FOXQ1 expression in rectal cancer group(r=-0.762,P=0.000);the 3-year survival rate of patients with serum miR-24-3 p increase rate>48.15% after operation was significantly higher than that of patients with increase rate<48.15%(P<0.05),the 3-year survival rate of patients with serum FOXQ1 decrease rate>33

关 键 词：直肠癌 miR-24-3p 叉头框蛋白Q1 

分 类 号：R735.3[医药卫生—肿瘤]