Ghrelin对人卵巢癌细胞株HO-8910 miR-1/Bcl-2表达的影响  

Effect of Ghrelin on expression of miR-1/Bcl-2 in human ovarian cancer cell line HO-8910

作　　者：刘瑞平 白瑞霞 赵鹏伟[2] LIU Rui-ping;BAI Rui-xia;ZHAO Peng-wei(Department of Clinical Laboratory,Inner Mongolia People＇s Hospital,Hohhot 010017,Inner Mongolia Autonomous Region,China)

机构地区：[1]内蒙古自治区人民医院检验科,内蒙古呼和浩特010017 [2]内蒙古医科大学,内蒙古呼和浩特010059

出　　处：《中国生物制品学杂志》2018年第11期1227-1229,共3页Chinese Journal of Biologicals

基　　金：内蒙古自治区自然科学基金项目(2016MS0888)

摘　　要：目的探讨Ghrelin对人卵巢癌细胞株HO-8910的调控作用。方法分别将400、500、600、700、800 ng/mL Ghrelin与HO-8910细胞一同孵育24 h,MTT法检测Ghrelin对细胞增殖的影响;将600 ng/mL Ghrelin作用于HO-8910细胞0、30、60 min后,定量PCR及Western blot法分别检测Ghrelin对细胞中miR-1以及Bcl-2、Caspase-12表达的影响。结果 600 ng/mL及以上浓度的Ghrelin作用24 h后,细胞抑制率明显升高,与400 ng/mL相比,差异有统计学意义(P <0. 05),Ghrelin抑制HO-8910细胞的增殖呈剂量依赖性;miR-1表达升高,Bcl-2表达下降,Caspase-12表达升高,作用30、60 min与0 min相比,作用60 min与30 min相比,差异均有统计学意义(P <0. 05)。结论Ghrelin能抑制人卵巢癌细胞株HO-8910增殖,Caspase-12表达的变化提示Ghrelin可能通过miR-1和Bcl-2调控卵巢癌细胞的增殖。Objective To investigate the regulatory effect of Ghrelin on human ovarian cancer cell line HO-8910.Methods HO-8910 cells were co-incubated with Ghrelin at concentrations of 400,500,600,700 and 800 ng/mL respectively,and determined for proliferation level by MTT assay. HO-8910 cells were treated with 600 ng/mL Ghrelin for 0,30 and 60 min,and determined for expression of miR-1 by quantitative PCR,and for those of Bcl-2 and Caspase-12 by Western blot. Results The inhibition rate of cells after treatment with Ghrelin at concentrations of not less than600 ng/mL for 24 h increased significantly,which showed significant difference with that after treatment with 400 ng/mL Ghrelin（P〈0. 05）,indicating a dose-dependent manner. The expression level of miR-1 increased,while that of Bcl-2 decreased,and that of Caspase-12 increased. However,the expression levels at various time points showed significant difference（each P〈0. 05）. Conclusion Ghrelin inhibited the proliferation of HO-8910 cells. The change of caspase-12 expression indicated that ghrelin might regulate the proliferation of HO-8910 cells through miR-1 and Bcl-2.

关 键 词：Gherlin 卵巢癌细胞 MIR-1 Bcl-2 

分 类 号：R737.31[医药卫生—肿瘤]