牙周炎对肥胖小鼠脂肪组织间质增生的影响  

Effects of periodontitis on stromal hyperplasia of adipose tissue in obese mice

作　　者：余挺[1] 赵莉[2] 章锦才 轩东英 YU Ting;ZHAO Li;ZHANG Jincai;XUAN Dongying(Department of Periodontology,Key Laboratory of Oral Medicine,Guangzhou Institute of Oral Disease,Stomatology Hospital of Guangzhou Medical University,Guangzhou 510140,China)

机构地区：[1]广州医科大学附属口腔医院.广州口腔疾病研究所.口腔医学重点实验室牙周科,广东广州510140 [2]中山大学光华口腔医学院.附属口腔医院修复科广东省口腔医学重点实验室,广东广州510055 [3]中国科学院大学存济医学院,北京100049 [4]中国科学院大学存济医学院杭州口腔医院牙周科,浙江杭州310006

出　　处：《口腔医学》2018年第11期966-969,共4页Stomatology

基　　金：国家自然科学基金(81700985;81371151;81470750);广州市卫生和计划生育科技项目(20181A011102)

摘　　要：目的荧光双染法探索牙周炎对肥胖机体脂肪组织间质增生的影响。方法建立饮食诱导型肥胖(饲料诱导16周)与牙周炎(结扎5 d)的复合小鼠模型。DAPI、Di I分别染色睾周脂肪组织的胞核和胞膜,分析肥胖、牙周炎对有核细胞数的效应。结果与苏木素-伊红、甲苯胺蓝染色相比,DAPI-DiI双染可清晰标记脂肪组织的胞核和脂肪细胞轮廓。肥胖显著上调脂肪组织的有核细胞数(P<0.05)。牙周炎对有核细胞数无显著影响(P>0.05)。结论短期牙周感染对肥胖相关的脂肪组织间质增生无明显影响。DAPI-DiI荧光双染有利于脂肪组织学分析包括有核细胞计数。Objective To explore effects of periodontitis on stromal hyperplasia of adipose tissue in obese individuals using DAPI-Dil double-staining. Methods Diet-induced obesity （16 weeks on diet） and periodontitis （5-day ligation） combined mouse models were established. Cell nuclei and membrane in epididymal adipose tissue were stained by DAPI and DiI, respectively, followed by analysis on effects of obesity and periodontitis on nucleated cell number. Results DAPI-DiI double-staining clearly demonstrated cell nuclei and adipocyte outline in adipose tissue compared to hematoxylin and eosin and toluidine blue staining methods. Nucleated cell number was significantly enhanced by obesity （P〈0.05）, but not obviously affected by periodontitis （P〉0.05）. Conclusion Short-term periodontal infection has no significant impact on obesity-related stromal hyperplasia in adipose tissue. DAPI-DiI double-staining may be helpful for adipose tissue histology including nucleated cell counting.

关 键 词：肥胖 牙周炎 脂肪组织 有核细胞 荧光染色 

分 类 号：R781.42[医药卫生—口腔医学]