光动力疗法对口腔生物膜致龋病变形链球菌及远缘链球菌作用研究  

Effect of Photodynamic Therapy on Streptococcus Mutans and Streptococcus Mutans in Dental Caries Induced by Oral Biofilm

作　　者：陶亚东 柳雪[2] 孙继红 王静[1] 霍峰[1] Tao Yadong;Liu Xue;Sun Jihong;Wang Jing;Huo Feng(l Department of Dental,The affiliated Hospital of Chengde Medical University,Chengde,Hebei 067000,Chin;Chengde Medical University,Chengde,Hebei 067000,China)

机构地区：[1]承德医学院附属医院口腔科,河北承德067000 [2]承德医学院,河北承德067000

出　　处：《应用激光》2018年第5期884-890,共7页Applied Laser

摘　　要：目的:体外构建符合人体生理环境的龋齿模型,探讨光动力疗法(Photodynamic therapy,PDT)防龋过程中,PDT对口腔生物膜中致龋菌变形链球菌以及远缘链球菌的作用;分析PDT防龋不同剂量的光敏剂以及激光能量对龋齿菌斑抑菌效果的影响,为防龋的临床实践提供实验依据。方法:通过体外远缘链球菌和变形链球菌体外抑菌实验,选择合适的光敏剂和激光剂量;选用变形链球菌以及远缘链球菌在体外构建符合人体生理环境的龋齿模型,并对人工龋模型中口腔生物膜生长曲线进行检测,以及釉质块表面显微硬度测定;检测HMME-PDT对细菌生长活力以及产酸的影响,采用显微硬度分析龋齿表面硬度变化,探讨PDT防龋的作用机制。结果:通过在体外对变形链球菌以及远缘链球菌的培养实验,选定HMME-PDT防龋的最佳HMME剂量为50μg/ml,激光光照剂量为78J/cm2;将牙釉质与致龋菌的共培养成功构建人工龋模型,人工龋齿组釉质表面显微硬度显著低于致龋前,差异有统计学意义(P<0.05);口腔生物膜内致龋菌的生成曲线在第3天达到平台期;PDT防龋可有效的抑制人工龋口腔生物膜中远缘链球菌以及变形链球菌的生长活力和产酸能力,相比于阴性对照组,差异有统计学意义(P<0.05),从而维持人工龋釉质块表面显微硬度,并显著高于其他组,差异有统计学意义(P<0.05);在大鼠龋齿模型中,PDT治疗能够显著改善大鼠牙龈上皮组织,骨组织等的异常。结论:选择合适的光敏剂剂量以及最佳的激光光照剂量,可有效的抑制人工龋口腔生物膜中远缘链球菌以及变形链球菌的生长活力,抑制其产酸能力,从而防止釉质的表面显微硬度进一步被破坏,HMME-PDT在龋齿的治疗中有着重要的临床价值。Objective： to construct the model of human dental caries physiological environment in vitro study of photodynamic therapy （Photodynamic therapy, PDT） in the process of PDT on dental caries, oral biofilm cariogenie bacteria Streptococcus and effect of Streptococcus sobrinus deformation; analysis of the influence of PDT with different doses of anti caries agent and light sensitive laser energy on the antibacterial effect of dental caries plaque. To provide the experimental basis for the clinical practice of dental caries prevention. Methods： the in vitro antibacterial experiment of Streptococcus sobrinus and deformation in vitro, select the appropriate dose of photosensitizer and laser; selection of Streptococcus mutans and Streptococcus sobrinus constructed in vitro with caries model of human physiological environment, and to detect the oral biofilm growth curve of artifi-cial caries model, and enamel surface microhardness determination; influence on detection of HMME-PDT bacterial growth and acid production activity, analysis of the changes of surface hardness By the microhardness of dental caries, and to explore the mechanism of PDT against dental caries. Results： through this experiment in vitro of Streptococcus mutans and Streptococcus sobrinus culture experiments, the optimal dose of selected HMME PDT caries： HMME dose of 50 g/ml, the laser irradiation dose of 78 J/cm^2 ; the enamel and eariogenic bacteria were cultured successfully constructed artificial caries model, artificial caries group was significantly lower than that of enamel surface microhardness dental caries, the difference was statistically sig-nificant （P（0.05）; oral biofilm formation curve of cariogenic bacteria reached a plateau in third days. PDT can effectively in-hibit the artificial caries caries oral biofilm of Streptococcus mutans and Streptococcus sobrinus in growth vigor, and acid pro-duction capacity, compared to the negative control group had significant difference （P〈0.05）, so as to maintain the artificial c

关 键 词：光动力学疗法 HMME-PDT 龋齿 口腔生物膜 

分 类 号：R781.1[医药卫生—口腔医学]