响应面优化脂肪酶催化合成三棕榈酸甘油酯  

Optimization of lipase-catalyzed synthesis of tripalmitin by response surface methodology

作　　者：徐坤华 王东铭 葛赞 史立文 计晓黎 雷小英 李伏益 耿二欢 XU Kunhua;WANG Dongming;GE Zan;SHI Liwen;JI Xiaoli;LEI Xiaoying;LI Fuyi;GENG Erhuan(Zanyu Technology Group Co.,Ltd.,Hangzhou 310030,China;Zhejiang Gongzheng Inspection Center Co.,Ltd.,Hangzhou 310030,China)

机构地区：[1]赞宇科技集团股份有限公司,杭州310030 [2]浙江公正检验中心有限公司,杭州310030

出　　处：《中国油脂》2018年第11期105-109,共5页China Oils and Fats

摘　　要：以单因素实验为基础,采用响应面法优化脂肪酶Cal B immo Plus在无溶剂体系中催化棕榈酸和甘油酯化反应合成三棕榈酯甘油酯工艺。结果表明:最佳合成工艺条件为反应时间12. 8 h、底物摩尔比2. 9∶1、反应温度75℃、加酶量4. 4%,在此条件下,酯化率和甘三酯含量分别为95. 44%和85. 75%。Based on single factor experiment, the synthesis process of tripalmitin （PPP） from palmitic acid and glycerol by CalB immo Plus lipase catalysis in a solvent free system was optimized by response surface methodology. The results showed that the optimal synthesis process conditions were obtained as follows： reaction time 12.8 h, molar ratio of palmitic acid to glycerol 2.9： 1, reaction temperature 75 ℃ and enzyme dosage 4.4%. Under these conditions, the esterification rate and the triglycerol content were 95.44% and 85.75%, respectively.

关 键 词：脂肪酶CalB immo PLUS 三棕榈酸甘油酯 响应面法 酯化 

分 类 号：Q814.9[生物学—生物工程] O621.3[理学—有机化学]