两种冷冻方法冻融人卵巢组织异种移植后血管生成素-2表达变化的比较  

Comparison of angiopoietin-2 expression on thawed and heteroplastic human ovarian tissues between two freezing methods

作　　者：张娜[1] 李微[1] 乜照燕[1] 张辉[2] 郝雅丽[1] 唐岚[1] ZHANG Na;LI Wei;NIE Zhao-yan;ZHANG Hui;HAO Ya-li;TANG Lan(Department of Reproductive Medicine,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;Department of Obstetrics and Gynecology,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院生殖医学科,石家庄050011 [2]河北医科大学第四医院妇产科,石家庄050011

出　　处：《解剖学报》2018年第6期825-829,共5页Acta Anatomica Sinica

基　　金：河北省重点研发计划(16277766D)

摘　　要：目的通过检测血管生成素-2(Ang-2)的表达,探讨两种冷冻方法对人卵巢组织的冻融及异种移植后的效果。方法共收集16例人卵巢组织标本,每例分成2份,其中1份应用程序化冷冻方法进行冷冻,另1份应用玻璃化冷冻方法进行冷冻。解冻后分别移植到去卵巢的雌性裸鼠颈部皮下。32只SCID裸鼠随机分为两组:A组16只,移植以程序化方法冷冻复苏的人卵巢组织;B组16只,移植以玻璃化方法冷冻复苏的人卵巢组织。解冻后及移植6周后观察两组卵巢组织中始基卵泡的存活及颗粒细胞Ang-2的表达情况。结果解冻后,A组Ang-2的阳性率明显高于B组(P<0.05),始基卵泡正常率两组相比差别不明显(P>0.05)。移植后,始基卵泡正常率及Ang-2的阳性率两组相比均无明显差别(P>0.05)。解冻后和移植后相比,两组的Ang-2阳性率及A组的始基卵泡正常率差别均不明显(P>0.05),B组移植后始基卵泡正常率明显低于解冻后(P<0.05)。结论与玻璃化冷冻相比,程序化冷冻对人卵巢组织始基卵泡颗粒细胞Ang-2的表达影响更小。将解冻后的人卵巢组织进行异种移植不影响Ang-2的表达。Objective To investigate the effect of thawing and xenotransplantation of two kinds of cryopreservation method on human ovarian tissue by detecting the expression of angiopoietin-2 （ Ang-2）. Methods Sixteen cases of human ovarian tissue specimens were collected, and each case was divided into two parts. One part was cryopreserved by programmed freezing and the other one was cryopreserved by vitrification. Thirty-two SCID nude mice were divided into two groups： group A were transplantated the thawed human ovarian tissue frozen by programming freezing, and group B, by vitrification. After thawing and 6 weeks after transplantation, the survival rate of the primordial follicles and the expression of Aug-2 in the granulosa cells were observed in the two groups. Results After thawing, the positive rate of Ang-2 in group A was significantly higher than that in group B （P 〈 0.05） , and there was no significant difference in the normal follicular rate between the two groups （ P 〉 0.05 ）. After transplantation, there were no significant differences in the survival rate of primordial follicles and the positive rate of Ang-2 between the two groups （ P 〉 0.05 ）. There were no significant differences in the positive rate of Aug-2 （ P 〉 0.05） in two groups and there was no significant difference in the survival rate of primordial follicles in group A between after thawing and after transplantation. But in group B, the survival rate of primordial follicles after transplantation was lower than after thawing （P 〈 0. 05 ）. Conclusion Programmed freezing had less effect on the expression of Ang-2 in the primordial follicle granulosa cells of the human ovarian tissue than vitrification. The heterograft of thawed human ovarian tissue does not affect the expression of Aug-2.

关 键 词：卵巢组织冷冻 程序化冷冻 玻璃化冷冻 血管生成素-2 免疫组织化学 裸鼠 

分 类 号：R361[医药卫生—病理学] R711[医药卫生—基础医学]