DKK3在胃癌组织中的甲基化表达及其临床意义  

The methylation expression and clinical significance of DKK3 in gastric cancer

作　　者：宋国栋[1] 王力[1] 沈洪[1] 李盟[1] 翟儒钧[1] 牛远杰[1] Song Guodong;Wang Li;Shen Hong;Li Meng;Zhai Rujun;Niu Yuanjie(Department of Gastrointestinal Surgery,the Second Hospital of Tianjin Medical University,Tianjin 300211,China)

机构地区：[1]天津医科大学第二医院胃肠外科,300211

出　　处：《中华普通外科杂志》2018年第11期935-937,共3页Chinese Journal of General Surgery

基　　金：天津市卫生和计划生育委员会科技基金资助项目（2015KZ096）

摘　　要：目的探讨胃癌组织中DKK3基因的mRNA表达、其启动子区甲基化状况及其与胃癌发生发展的关系。方法应用甲基化特异性PCR检测76例配对的胃癌和癌旁组织中DKK3基因启动子甲基化状态，再采用RT-PCR检测该基因的mRNA表达。结果76例胃癌组织中DKK3mRNA表达率低于癌旁组织（43％比60％，X2=5．663，P=0．017）。在癌旁组织中，有29例出现DKK3基因启动子甲基化，而在胃癌组织中有44例出现甲基化（38％比58％，X2=19．405，P=0．000）。胃癌组织中DKK3基因启动子甲基化表达与胃癌患者的肿瘤浸润深度（P=0．014）、TNM分期（P=0．032）、病理类型（P=0．016）及淋巴结转移（P=0．007）密切相关。DKK3基因启动子甲基化与其mRNA表达具有负相关性（X2=64．286，P=0．000）。结论胃癌组织中DKK3基因启动子的异常甲基化是其失活的重要机制．在胃癌的进展中发挥了重要作用。Objective To study the correlation between status of methylation of DKK3 gene promoter and expression of its mRNA in gastric cancer. Methods Methylation of DKK3 gene promotor and its mRNA expression in 76 gastric cancer specimens and paired adjacent non-cancerous tissues were detected by methylation-specific PCR（MSP）and RT-PCR respectively. Results The expression of DKK3 mRNA in gastric cancer tissues was lower than that of adjacent non-cancerous tissues （43% vs. 60% , X2 = 5. 663, P = 0. 017 ）. 29 cases had methylation of DKK3 gene promoter for adjacent non-cancerous tissues. In gastric cancer tissues,however,44 cases had methylation of DKK3 gene promoter （38% vs. 58%, A2 = 19. 405, P =0. 000）. There were significant difference in depth of invasion （P =0. 014）, TNM stage （P =0. 032）, pathological grading （ P = 0. 016 ） and lymphatic metastasis （ P = 0. 007 ） . There was distinct negative correlation between methylation of DKK3 gene promoter and expression of its mRNA （X2 = 64. 286, P = 0. 000）. Conclusions Abnormal methylation of DKK3 gene promoter is an important mechanism of its inactivation, and might play an important role in carcinogenesis and progression of gastric cancer.

关 键 词：胃肿瘤 DNA甲基化 病理学 临床 DKK3 

分 类 号：R735.2[医药卫生—肿瘤]