长链非编码RNA-MIAT促进结直肠癌细胞的增殖及其机制  

Long non-coding RNA-MIAT promotes proliferation of colorectal cancer by regulating Wnt/β-catenin pathway

作　　者：刘永武[1] 章周海 邵拥军 Liu Yongwu;Zhang Zhouhai;Shao Yongjun(Deaprtrnent of General Surgery,Anhui Provincial Cancer Hospital,Hefei 233004,China)

机构地区：[1]安徽省肿瘤医院安徽省立医院(西区)综合外科,合肥233004

出　　处：《中华普通外科杂志》2018年第11期938-941,共4页Chinese Journal of General Surgery

摘　　要：目的分析MIAT在结直肠癌组织中的表达情况并探讨其分子机制。方法用PCR方法检测17例结直肠癌组织及癌旁组织中、结直肠癌细胞系和人正常结直肠黏膜细胞中MIATmRNA表达水平。在HCT-116细胞中过表达或敲降MIAT，检测细胞增殖活力和细胞周期。用Westernblot检测β-catenin表达水平，荧光素酶报告基因检测MIAT对β-catenin启动子转录水平的影响．RT-qPCR检测C-myc、CCND1、CIM4和Oct3／4mRNA的表达水平。结果17例结直肠癌组织中的MIAT表达水平高于癌旁组织，差异有统计学意义（t=4．093，P〈0．05）；高表达MIAT可以促进HCT．116细胞增殖，降低G0／G1期细胞的比例，激活Wnt/β-catenin通路，增加β-catenin启动子转录活性及其下游靶基因mRNA的表达水平，差异均有统计学意义（均P〈0．05）。结论MIAT是结直肠癌的促癌基因。可能通过激活Wnt/β-catenin通路发挥肿瘤促进作用。Objective To analyze the expression of MIAT in colorectal cancer and explore its molecular mechanism. Methods The expression of MIAT mRNA in colorectal cancer tissues and paracancerous tissues, colorectal cancer cell lines and human normal colorectal mucosa cells was detected by RT-qPCR. HCT-116 cells were transfected with MIAT overexpression vector and infected with knockdown lentivirus, cell proliferation and cell cycle were detected, Western blot was used to analyze the β-catenin protein levels, luciferase assay was used to detect β-catenin promoter transcription level, C-myc, CCND1, CD44 and Oct3/4 mRNA levels was measured by RT-qPCR. Results The expression of MIAT in eolorectal cancer tissues was signifieantly higher than that in adjacent tissues （ t = 4. 093, P 〈 0. 05 ） . Overexpression of MIAT could promote the proliferation of HCT-116 cells, decrease the rate of G0/G1 phase, activate Wnt/β-catenin pathway and increase its downstream target gene mRNA expression levels （ all P 〈 0. 05 ）. Knockdown MIAT shows the inhibition effect. Conclusions MIAT is a tumor-promoting gene of colorectal cancer and may play a role in tumor promotion by activating Wnt/β-catenin pathway.

关 键 词：结直肠肿瘤 RNA  信使 WNT/Β-CATENIN通路 

分 类 号：R735.34[医药卫生—肿瘤]