饲料花生四烯酸水平对珍珠龙胆石斑鱼幼鱼生长性能、抗氧化能力、血清生化指标以及肝脏和肌肉脂肪酸组成的影响  

Effects of Dietary Arachidonic Acid Level on Growth Performance,Antioxidant Ability,Serum Biochemical Parameters and Fatty Acid Composition in Liver and Muscle of Juvenile Hybrid Grouper(Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus)

作　　者：王成强 王际英 黄炳山 李宝山 孙永智 王晓艳 马长兴 WANG Chengqiang;WANG Jiying;HUANG Bingshan;LI Baoshan;SUN Yongzhi;WANG Xiaoyan;MA Changxing(Shandong Provincial Key Laboratory of Restoration for Marine Ecology,Shandong Marine Resource andEnvironment Research Institute,Yantai 264006,China;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]山东省海洋资源与环境研究院山东省海洋生态修复重点实验室,烟台264006 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《动物营养学报》2018年第9期3567-3580,共14页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省重点研发计划(2016GSF115005);山东省科学自然基金(ZR2015CQ023);烟台市科技发展计划(2016ZH068);国家海洋公益性行业科研专项(201505022)

摘　　要：本试验旨在研究饲料花生四烯酸(ARA)水平对珍珠龙胆石斑鱼幼鱼生长性能、抗氧化能力、血清生化指标以及肝脏和肌肉脂肪酸组成的影响。通过在基础饲料中添加ARA纯化油,制成ARA水平分别为0.04%、0.17%、0.35%、0.66%、1.29%和2.16%(干物质基础)的等氮等脂的试验饲料(分别命名为S1、S2、S3、S4、S5和S6)。将上述饲料投喂初始体重为(23.77±0.98)g的珍珠龙胆石斑鱼幼鱼,每种饲料设3个重复,每个重复投喂30尾鱼,试验期为8周。结果表明:1)特定生长率(SGR)和饲料效率(FE)随饲料ARA水平的升高均呈先升高后降低的趋势,且SGR和FE均在S4组有最大值,均显著高于S1组(P<0.05)。全鱼和肝脏粗脂肪含量均在S3组最低,显著低于S5和S6组(P<0.05)。2)S4组肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和总抗氧化能力(T-AOC)与S3组差异不显著(P>0.05),但显著高于S1与S6组(P<0.05)。S3与S4组肝脏丙二醛(MDA)含量显著低于S1和S6组(P<0.05)。3)血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)和碱性磷酸酶(AKP)活性均在S4组最低,且显著低于S1与S6组(P<0.05)。4)肝脏和肌肉中C20∶3n-6和C20∶4n-6含量随饲料ARA水平的提高而显著升高(P<0.05),而C18∶3n-6、C20∶5n-3和C22∶6n-3含量则随之有不同程度的下降。由此得出,饲料中适宜水平(0.35%~0.66%)的ARA能够促进珍珠龙胆石斑鱼幼鱼的生长,提高抗氧化能力与肝脏健康水平,以SGR与FE作为评价指标,经折线模型回归分析得出珍珠龙胆石斑鱼幼鱼饲料中ARA的适宜水平分别为饲料干重的0.45%和0.56%。An 8-week feeding experiment was conducted to evaluate the effects of dietary arachidonic acid（ARA） level on growth performance, antioxidant ability, serum biochemical parameters and fatty acid com-position in liver and muscle of juvenile hybrid grouper （Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ）.Six isonitrogenous and isoenergetic diets were formulated with the ARA level of 0. 04%, 0. 17%, 0. 35%,0.66%, 1.29% and 2.16% （ dry matter basis） （ named S1, S2, S3, S4, S5 and S6, separately） , respective-ly. Each diet had 3 replicates and each replicate cultured 30 juvenile hybrid grouper with an initial body weightof （23.77±0.98） g. The results showed as follows： 1） the specific growth rate （ SGR） and feed efficiency（FE） increased at first, and then decreased with the increase of dietary ARA level, which reaching their peaksat S4 group （P〈0.05） , and significantly higher than S1 group （P〈0.05） . The body and liver ether extractcontent of S3 group was the lowest, and significantly lower than that of S5 and S6 groups （P〈0.05） . 2） Theactivities of superoxide dismutase （SOD） and catalase （CAT） , total antioxidant capacity （T-AOC） in liver ofS4 group were no significant differences with S3 group （P〉0.05） , but significantly higher than those of S1and S6 groups （P〈0.05） . The malondialdehyde （MDA） content in liver of S3 and S4 groups was significant-ly lower than that of S1 and S6 groups （ P〈0.05） . 3） The lowest values of the activities of aspartate amin-otransferase （AST） , cereal third transaminase （ALT） and alkaline phosphatase （AKP） in serum were foundin S4 group, and significantly lower than those of S1 and S6 groups （P〈0.05） . 4） The contents of C20∶3n-6and C20∶4n-6 in liver and muscle were significantly increased with the increase of dietary ARA level, but thecontents of C18∶3n-6, C20∶5n-3 and C22 ∶6n-3 were decreased with different extents. These results indicatethat optimal dietary

关 键 词：珍珠龙胆石斑鱼 花生四烯酸 生长性能 血清生化指标 脂肪酸组成 

分 类 号：S936[农业科学—渔业资源]