饲粮中添加高剂量茶多酚对产蛋后期蛋鸡生产性能、蛋品质和脂质代谢的影响  

Effects of Adding High Doses of Tea Polyphenols into Diets on Performance,Egg Quality and Lipid Metabolism of Laying Hens during Later Laying Period

作　　者：何俊金 王建萍[1] 丁雪梅[1] 曾秋凤[1] 白世平[1] 张克英[1] HE Junjin;WANG Jianping;DING Xuemei;ZENG Qiufeng;BAI Shiping;ZHANG Keying(Key Laboratory for Animal Disease-Resistance Nutrition and Feed of Ministry,Key Laboratory forAnimal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学动物营养研究所动物抗病营养教育部重点实验室动物抗病营养与饲料农业部重点实验室,成都611130

出　　处：《动物营养学报》2018年第11期4601-4610,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家科技支撑计划(2014BAD13B04);四川省蛋鸡产业链项目(2014NZ0002);四川省科创饲料产业技术研究院(2013NZ0054)

摘　　要：本试验旨在研究饲粮中添加高剂量茶多酚对产蛋后期蛋鸡生产性能、蛋品质、血清脂质代谢指标和肝脏脂质代谢相关基因表达的影响。选用480羽65周龄罗曼粉壳蛋鸡作为试验动物,随机分成4组,每组8个重复,每个重复15羽。对照组饲喂基础饲粮,3个试验组分别饲喂在基础饲粮中添加666、1 333和2 666 mg/kg茶多酚(纯度为63.58%,其中儿茶素含量为40.20%)的试验饲粮,预试期2周,正试期9周。结果显示:1)与对照组相比,添加2 666 mg/kg茶多酚显著降低产蛋率(第1～3周、第4～6周和第1～9周)、平均日采食量(第1～3周、第4～6周、第7～9周和第1～9周),显著升高料蛋比(第1～3周和第4～6周); 1 333和2 666 mg/kg茶多酚组的平均蛋重在第1～3周、第4～6周和第1～9周显著低于对照组(P<0.05)。2)与对照组相比,饲粮添加2 666 mg/kg茶多酚显著提高第2周哈夫单位(P<0.05),且显著降低第2周蛋壳比率(P<0.05);饲粮添加1 333 mg/kg茶多酚显著提高第4周哈夫单位(P<0.05),且显著降低第2周蛋壳比率(P<0.05);饲粮添加666、1 333和2 666 mg/kg茶多酚均显著降低第9周蛋黄胆固醇含量(P<0.05);饲粮添加666、1 333和2 666 mg/kg茶多酚对蛋壳强度、蛋黄颜色、蛋黄比率、蛋白比率及蛋黄脂肪酸组成均无显著影响(P>0.05)。3)与对照组相比,饲粮添加666、1 333和2 666 mg/kg茶多酚显著降低血清总胆固醇、低密度脂蛋白胆固醇含量(P<0.05),饲粮添加1 333和2 666 mg/kg茶多酚显著降低血清甘油三酯含量(P<0.05)。4)与对照组相比,饲粮添加666、1 333和2 666 mg/kg茶多酚显著提高肝脏腺苷酸激活蛋白激酶(AMPK) mRNA相对表达量(P<0.05)。由此可知,饲粮中添加高剂量(1 333和2 666 mg/kg)的茶多酚会降低蛋鸡产蛋后期的生产性能,但可以提高鸡蛋的哈夫单位,降低血清总胆固醇和低密度脂蛋白胆固醇含量及蛋黄胆固醇含量,并上调肝脏AMPK基因的表达。This experiment was conducted to study the effects of adding high doses of tea polyphenols（TP）into diets on performance,egg quality and serum lipometabolism parameters and hepatic lipometabolism-related gene expression of laying hens during later laying period.A total of 480 Lohmann laying hens（65-week-old）were randomly allotted into 4 groups with 8 replicates per group and 15 hens per replicate.The laying hens in control group were fed a basal diet,and those in experimental groups were fed the basal diets supplemented with 666,1 333 and 2 666 mg/kg TP（its purity reached 63.58%,and the content of catechin in it was40.20%）,respectively.There was a pretrial period of 2 weeks followed by an experimental period of 9 weeks.The results showed as follow s：1） compared with the control group,adding 2 666 mg/kg TP significantly decreased the laying rate of week 1 to 3,4 to 6 and 1 to 9（P〈0.05）,significantly decreased the average daily feed intake of week 1 to 3,4 to 6,7 to 9 and 1 to 9（P〈0.05）,and significantly increased the feed/egg of week 1 to 3 and 4 to 6（P〈0.05）;adding 1 333 and 2 666 mg/kg TP significantly decreased the average egg weight of week 1 to 3,4 to 6 and 1 to 9（P〈0.05）.2） Compared with the control group,adding2 666 mg/kg TP significantly increased the Haugh unit of week 2（P〈0.05）,and significantly decreased the eggshell ratio of week 2（P〈0.05）;adding 1 333 mg/kg TP significantly increased the Haugh unit of week 4（P〈0.05）,and significantly decreased the eggshell ratio of week 2（P〈0.05）;adding 666,1 333 and2 666 mg/kg TP significantly decreased egg yolk cholesterol content of week 9（P〈0.05）;adding 666,1 333 and 2 666 mg/kg TP did not significantly affect the eggshell strength,egg yolk color,egg yolk ratio,albumen ratio and egg yolk fatty acid composition（P〉0.05）.3） Compared with the control group,adding 666,1 333 and 2 666 mg/kg TP significantly decreased the serum total cholesterol（TC） and low density lipoprotein cholesterin�

关 键 词：茶多酚 蛋鸡 生产性能 蛋品质 脂质代谢 

分 类 号：S816[农业科学—饲料科学]