转化生长因子-β_1对人牙周膜干细胞生物学行为的影响  

Effect of transforming growth factor-β_1 on biological behavior of human periodontal ligament stem cells

作　　者：龙晏 邱申彩 李淑慧[1] 舍玉秀 王娜[1] 陈晓燕 吴佩玲[1] LONG Yan;QIU Shencai;LI Shuhui;SHE Yuxiu;WANG Na;CHEN Xiaoyan;WU Peiling(Department of Stomatology,the Second Affiliated Hospital of Xinjiang Medical University ~ Urumqi 830063,China)

机构地区：[1]新疆医科大学第二附属医院口腔科,乌鲁木齐830063

出　　处：《中华实用诊断与治疗杂志》2018年第11期1044-1046,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(81460103)

摘　　要：目的探讨转化生长因子-β1(transforming growth factor-β1,TGF-β1)对人牙周膜干细胞(periodontal ligament stem cells,PDLSCs)增殖和迁移能力的影响及作用机制。方法对数生长期人PDLSCs随机分为TGF-β1组(培养液+10μg/L TGF-β1)和对照组(培养液),采用CCK-8法检测2组培养第1、2、3、4、5、6、7天细胞增殖能力;培养48h,采用Transwell小室法检测2组细胞迁移能力,Western blot法检测2组Notch1胞内段(Notch1intracellular domain,NICD)蛋白相对表达量。结果 TGF-β1组培养第1天细胞吸光度值(0.45±0.02)与对照组(0.45±0.03)比较差异无统计学意义(P>0.05),培养第2、3、4、5、6、7天细胞吸光度值(0.81±0.08、1.82±0.07、2.14±0.09、2.49±0.11、2.69±0.12、2.78±0.08)均高于对照组(0.61±0.04、1.17±0.05、1.47±0.10、1.99±0.11、2.20±0.03、2.29±0.08)(P<0.05);培养48h,TGF-β1组迁移细胞数目[(61.40±2.30)%]较对照组[(31.80±1.48)%]多,NICD蛋白相对表达量(0.49±0.01)较对照组(0.35±0.01)高(P<0.05)。结论 TGF-β1可激活Notch1信号通路,促进人PDLSCs增殖和迁移。Objective To investigate the effect of transforming growth factor-β1（TGF-β1）on the proliferation and migration of human periodontal ligament stem cells（PDLSCs）and its mechanism.Methods PDLSCs in logarithmic growth phase were randomly divided into TGF-β1 group（culture medium＋10μg/L TGF-β1）and control group（culture medium）.The proliferation of PDLSCs was detected by CCK-8 method on the 1 st,2 nd,3 rd,4 th,5 th,6 th and 7 th days of culture in two groups.The migration ability of PDLSCs was detected by Transwell method in 48 hof culture,and the relative expression of Notch1 intracellular domain（NICD）protein was detected by Western blot method.Results On the1 st day of culture,there was no significant difference in the proliferation optical density value between TGF-β1 group（0.45±0.02）and control group（0.45±0.03）（P0.05）,and the optical density values on the 2 nd,3 rd,4 th,5 th,6 th and 7 th days of culture were significantly higher in TGF-β1 group（0.81±0.08,1.82±0.07,2.14±0.09,2.49±0.11,2.69±0.12,2.78±0.08）than those in control group（0.61±0.04,1.17±0.05,1.47±0.10,1.99±0.11,2.20±0.03,2.29±0.08）（P0.05）.The number of migrating cells was significantly larger in TGF-β1 group（（61.40±2.30）%）than that in control group（（31.80±1.48）%）,and the relative expression of NICD protein was significantly higher in TGF-β1 group（0.49±0.01）than that in control group（0.35±0.01）in 48 hof culture（P0.05）.Conclusion TGF-β1 can promote the proliferation and migration of human PDLSCs by activating the Notch1 signaling pathway.

关 键 词：人牙周膜干细胞 转化生长因子-Β1 细胞增殖 细胞迁移 

分 类 号：R781[医药卫生—口腔医学]