过表达GINS2基因对NB4细胞增殖的影响  

Proliferation effect of NB4 cells induced by over-expression GINS2

作　　者：高艳军[1] 邢志伟[1] 杜月菊[1] 徐美丽[1] 李刚[1] 王世博[2] GAO Yan-jun;XING Zhi-wei;DU Yue-ju(The Clinical Laboratory of Hebei Chest Hospital,Shijiazhuang 050041,China;Department of Infection Management,The Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北省胸科医院检验科,河北石家庄050041 [2]河北医科大学第二医院感染控制处,河北石家庄050000

出　　处：《中国实验诊断学》2018年第11期1935-1938,共4页Chinese Journal of Laboratory Diagnosis

基　　金：河北省医学科学研究重点课题计划项目(20150602)

摘　　要：目的利用腺病毒载体Ad-GINS2感染NB4细胞,观察其对NB4细胞增殖的影响。方法通过AdGINS2感染NB4细胞,利用荧光显微镜观察NB4细胞的感染情况,利用PCR和Western blotting技术检测GINS2基因的转录和翻译水平;MTT方法检测NB4细胞增殖情况。结果 PCR检测结果显示,与空载组和未处理组相比,GINS2的表达水平明显增加(P<0.05);Western blotting技术检测结果显示,与空载组和未处理组相比,GINS2的表达水平明显增加(P<0.05);MTT结果显示,与未处理组和空载组相比,重组腺病毒Ad-GINS2能明显促进NB4细胞的增殖(P<0.05)。结论 Ad-GINS2成功感染NB4细胞并且促进NB4细胞增殖,为明确该基因在髓细胞白血病中的作用奠定了一定基础。Objective To investigate the effect on Proliferation of NB4 cell infected by Ad-GINS2.Methods NB4 cells were infected by Ad-GINS2 and using fluorescence microscope observe the infection effect of NB4 cells.PCR and Western blotting were used to detect the expression level of GINS2.MTT assay were used to detect the effect of cell proliferation in NB4 cells.Results GINS2 increased significantly using PCR technology compared with the untreated group and no-load group（P〈0.05）,GINS2 protein increased significantly using Western blotting technology compared with the untreated group and no-load group（P〈0.05）;MTT assay test showed that over-expression of GINS2 could stimulate proliferation of NB4 cells compared with the untreated group and no-load group（P〈0.05）.Conclusion AdGINS2 successfully infected NB4 cells and advanced the proliferation of NB4 cells,laid a certain foundation to clarify the role of GINS in myelogenous leukemia.

关 键 词：Ad-GINS2 NB4细胞 过表达 增殖 

分 类 号：R733[医药卫生—肿瘤]